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Literature summary extracted from

  • Phillippy, B.Q.; Ullah, A.H.J.; Ehrlich, K.C.
    Purification and some properties of inositol 1,3,4,5,6-pentakis phosphate 2-kinase from immature soybean seeds (1994), J. Biol. Chem., 269, 28393-28399.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.158 bovine serum albumin 0.0025%, increases activity of purified enzyme by 40% Glycine max
2.7.1.158 Triton X-100 0.01%, increases activity Glycine max
2.7.1.158 Tween 20 0.01%, increases activity Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.158 (NH4)2SO4 slightly inhibitory Glycine max
2.7.1.158 Ca2+ 20 mM, complete inhibition Glycine max
2.7.1.158 KCl strongly inhibitory at 200 mM Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.158 0.0023
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
Glycine max
2.7.1.158 0.0084
-
ATP
-
Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.158 Mg2+ maximal activity at 4 mM Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.158 52000
-
x * 52000, SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Glycine max the enzyme may be involved in both inositol hexakisphosphate formation in maturing seeds and ATP resynthesis in germinating seeds ADP + 1D-myo-inositol hexakisphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.158 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.158
-
Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.158 seed immature Glycine max
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.158 0°C, enzyme retains 50% of its activity after 6 weeks Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
Glycine max ADP + 1D-myo-inositol hexakisphosphate
-
?
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate the enzyme may be involved in both inositol hexakisphosphate formation in maturing seeds and ATP resynthesis in germinating seeds Glycine max ADP + 1D-myo-inositol hexakisphosphate
-
?
2.7.1.158 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
Glycine max ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.158 ? x * 52000, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.7.1.158 inositol 1,3,4,5,6-pentakis phosphate 2-kinase
-
Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.158 42
-
-
Glycine max

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.158 30 50 30°C: about 60% of maximal activity, 50°C: about 10% of maximal activity Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.158 6.8
-
-
Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.158 5.5 8 about 50% of maximal activity at pH 5.5 and pH 8.0 Glycine max