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Literature summary extracted from

  • Baker, B.J.; Dotzlaf, J.E.; Yeh, W.K.
    Deacetoxycephalosporin C hydroxylase of Streptomyces clavuligerus. Purification, characterization, bifunctionality, and evolutionary implication (1991), J. Biol. Chem., 266, 5087-5093.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.26 dithiothreitol
-
Streptomyces clavuligerus
1.14.11.26 glutathione reduced Streptomyces clavuligerus
1.14.20.1 dithiothreitol
-
Streptomyces clavuligerus
1.14.20.1 glutathione reduced Streptomyces clavuligerus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.26 5,5'-dithiobis-2-nitrobenzoic acid 1 mM, no residual activity Streptomyces clavuligerus
1.14.11.26 EDTA 0.5 mM, no residual activity Streptomyces clavuligerus
1.14.11.26 iodoacetic acid 1 mM, 19% residual activity Streptomyces clavuligerus
1.14.11.26 N-ethylmaleimide 1 mM, no residual activity Streptomyces clavuligerus
1.14.11.26 o-phenanthroline 0.5 mM, no residual activity Streptomyces clavuligerus
1.14.11.26 p-hydroxymercuribenzoate 0.1 mM, no residual activity Streptomyces clavuligerus
1.14.11.26 Penicillin N 1 mM, 62% inhibition, competitive with deacetoxycephalosporin C Streptomyces clavuligerus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.26 Fe2+ required, without Fe2+, less than 2% of maximum activity Streptomyces clavuligerus
1.14.11.26 additional information Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ Streptomyces clavuligerus
1.14.20.1 Fe2+ required, without Fe2+, less than 2% of maximum activity Streptomyces clavuligerus
1.14.20.1 additional information Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ Streptomyces clavuligerus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.26 Streptomyces clavuligerus
-
also has slight activity of EC 1.14.20.1, ring expansion reaction of penicillin N
-
1.14.20.1 Streptomyces clavuligerus
-
also has activity of EC 1.14.11.26, deacetoxycephalosporin hydroxylase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.26 3-exo-methylenecephalosporin C + 2-oxoglutarate + O2 37% of activity with deacetoxycephalosporin C Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2
-
?
1.14.11.26 deacetoxycephalosporin C + 2-oxoglutarate + O2
-
Streptomyces clavuligerus deacetylcephalosporin C + succinate + CO2
-
?
1.14.20.1 penicillin N + 2-oxoglutarate + O2 about 3%, compared to activity of EC 1.14.11.26, deacetoxycephalosporin hydroxylase Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2 + H2O
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.26 0.12
-
Penicillin N pH 7.3, 29°C Streptomyces clavuligerus