EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.26 | dithiothreitol | - |
Streptomyces clavuligerus | |
1.14.11.26 | glutathione | reduced | Streptomyces clavuligerus | |
1.14.20.1 | dithiothreitol | - |
Streptomyces clavuligerus | |
1.14.20.1 | glutathione | reduced | Streptomyces clavuligerus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.26 | 5,5'-dithiobis-2-nitrobenzoic acid | 1 mM, no residual activity | Streptomyces clavuligerus | |
1.14.11.26 | EDTA | 0.5 mM, no residual activity | Streptomyces clavuligerus | |
1.14.11.26 | iodoacetic acid | 1 mM, 19% residual activity | Streptomyces clavuligerus | |
1.14.11.26 | N-ethylmaleimide | 1 mM, no residual activity | Streptomyces clavuligerus | |
1.14.11.26 | o-phenanthroline | 0.5 mM, no residual activity | Streptomyces clavuligerus | |
1.14.11.26 | p-hydroxymercuribenzoate | 0.1 mM, no residual activity | Streptomyces clavuligerus | |
1.14.11.26 | Penicillin N | 1 mM, 62% inhibition, competitive with deacetoxycephalosporin C | Streptomyces clavuligerus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.26 | Fe2+ | required, without Fe2+, less than 2% of maximum activity | Streptomyces clavuligerus | |
1.14.11.26 | additional information | Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ | Streptomyces clavuligerus | |
1.14.20.1 | Fe2+ | required, without Fe2+, less than 2% of maximum activity | Streptomyces clavuligerus | |
1.14.20.1 | additional information | Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ | Streptomyces clavuligerus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.26 | Streptomyces clavuligerus | - |
also has slight activity of EC 1.14.20.1, ring expansion reaction of penicillin N | - |
1.14.20.1 | Streptomyces clavuligerus | - |
also has activity of EC 1.14.11.26, deacetoxycephalosporin hydroxylase | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.26 | 3-exo-methylenecephalosporin C + 2-oxoglutarate + O2 | 37% of activity with deacetoxycephalosporin C | Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 | - |
? | |
1.14.11.26 | deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetylcephalosporin C + succinate + CO2 | - |
? | |
1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | about 3%, compared to activity of EC 1.14.11.26, deacetoxycephalosporin hydroxylase | Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.26 | 0.12 | - |
Penicillin N | pH 7.3, 29°C | Streptomyces clavuligerus |