Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Thage, B.V.; Rattray, F.P.; Laustsen, M.W.; Ardo, Y.; Barkholt, V.; Houlberg, U.
    Purification and characterization of a branched-chain amino acid aminotransferase from Lactobacillus paracasei subsp. paracasei CHCC 2115 (2004), J. Appl. Microbiol., 96, 593-602.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.42 nutrition use of enzyme for development of flavor in cheese Lacticaseibacillus paracasei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 carboxymethoxylamine 1 mM, 20% residual activity Lacticaseibacillus paracasei
2.6.1.42 Co2+ 1 mM, 49% residual activity Lacticaseibacillus paracasei
2.6.1.42 Cu2+ 1 mM, 39% residual activity Lacticaseibacillus paracasei
2.6.1.42 Fe2+ 1 mM, 34% residual activity Lacticaseibacillus paracasei
2.6.1.42 Hg2+ 1 mM, 0% residual activity Lacticaseibacillus paracasei
2.6.1.42 hydroxylamine 1 mM, 3% residual activity Lacticaseibacillus paracasei
2.6.1.42 additional information not inhibitory: 4% NaCl, 1 mM iodoacetamide, 1 mM phenylmethylsulfonylfluoride Lacticaseibacillus paracasei
2.6.1.42 phenylhydrazine 1 mM, 0% residual activity Lacticaseibacillus paracasei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 41000
-
 1 * 41000, SDS-PAGE Lacticaseibacillus paracasei
2.6.1.42 50000
-
 gel filtration Lacticaseibacillus paracasei

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Lacticaseibacillus paracasei
-
 subsp. paracasei CHCC 2115
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42
-
 Lacticaseibacillus paracasei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 11.1
-
 pH 7.4, 37°C Lacticaseibacillus paracasei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Lacticaseibacillus paracasei 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxoglutarate 82% of the activity with L-isoleucine Lacticaseibacillus paracasei 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.42 L-leucine + 2-oxohexanoic acid 214% of the activity with 2-oxoglutarate Lacticaseibacillus paracasei 4-methyl-2-oxopentanoate + 2-aminohexanoic acid
-
 ?
2.6.1.42 L-leucine + 2-oxoisovaleric acid 135% of the activity with 2-oxoglutarate Lacticaseibacillus paracasei 4-methyl-2-oxopentanoate + 2-aminoisovaleric acid
-
 ?
2.6.1.42 L-methionine + 2-oxoglutarate 6% of the activity with L-isoleucine Lacticaseibacillus paracasei 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 ?
2.6.1.42 L-valine + 2-oxoglutarate 86% of the activity with L-isoleucine Lacticaseibacillus paracasei 3-methyl-2-oxobutanoate + L-glutamate
-
 ?
2.6.1.42 additional information no substrate: phenylalanine, tyrosine, tryptophan, aspartic acid, asparagine Lacticaseibacillus paracasei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 monomer 1 * 41000, SDS-PAGE Lacticaseibacillus paracasei
2.6.1.42 More N-terminal amino acid sequence Lacticaseibacillus paracasei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.42 43
-
-
 Lacticaseibacillus paracasei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 5.2
-
 and 4% NaCl, 13% of maximum activity Lacticaseibacillus paracasei
2.6.1.42 7.3
-
-
 Lacticaseibacillus paracasei