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Literature summary extracted from
- Dynamic kinetic resolution of amino acid amide catalyzed by D-aminopeptidase and alpha-amino-epsilon-caprolactam racemase (2005), J. Am. Chem. Soc., 127, 7696-7697.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.11.19 | synthesis | production of D-amino acids from L-amino acid amides by combination of alpha-amino-epsilon-caprolactam racemase, EC 5.1.1.15, and enzyme. Yield of conversion of L-alanine amid is more than 99% | Brucella anthropi |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.15 | expression in Escherichia coli | Achromobacter obae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.15 | 1.1 | - |
L-2-aminobutyric acid amide | - |
Achromobacter obae |  |
| 5.1.1.15 | 2.5 | - |
L-Ala | - |
Achromobacter obae | |
| 5.1.1.15 | 3.4 | - |
D-Ala | - |
Achromobacter obae | |
| 5.1.1.15 | 3.5 | - |
D-alpha-Amino-epsilon-caprolactam | - |
Achromobacter obae | |
| 5.1.1.15 | 10 | - |
L-alpha-amino-epsilon-caprolactam | - |
Achromobacter obae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.19 | Brucella anthropi | - |
recombinant protein | - |
| 5.1.1.15 | Achromobacter obae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.15 | recombinant | Achromobacter obae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.15 | D-Ala | - |
Achromobacter obae | L-Ala | - |
r | |
| 5.1.1.15 | L-2-aminobutyric acid amide | - |
Achromobacter obae | D-2-aminobutyric acid amide | - |
r | |
| 5.1.1.15 | L-alpha-Amino-epsilon-caprolactam | - |
Achromobacter obae | D-alpha-Amino-epsilon-caprolactam | - |
? | |
| 5.1.1.15 | additional information | no racemization activity is observed with dipeptides or amino acid derivatives, such as L-Ala-D-Ala, D-Ala-L-Ala, L-alanylglycine, L-phenylglycine, or L-alanine methylester | Achromobacter obae | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.1.15 | ACL racemase | - |
Achromobacter obae |
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Release 2023.1 (January 2023)
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