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Literature summary extracted from

  • De Vries, T.; Storm, J.; Rotteveel, F.; Verdonk, G.; Van Duin, M.; Van den Eijnden, D.H.; Joziasse, D.H.; Bunschoten, H.
    Production of soluble human alpha3-fucosyltransferase (FucT VII) by membrane targeting and in vivo proteolysis (2001), Glycobiology, 11, 711-717.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.214 pharmacology the enzyme is a target for rational inhibitor design for medication in the treatment of rheumatoid arthritis Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.214 expression of the soluble enzyme, by constructing a fusion enzyme amino acid residues 1-63 of a mammalian enzyme fused to the stem and the catalytic region of residues 39-342 of the enzyme, in CHO cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.214 ADP slight inhibition at 3-5 mM Homo sapiens
2.4.1.214 CDP slight inhibition at 3-5 mM Homo sapiens
2.4.1.214 Cu2+
-
Homo sapiens
2.4.1.214 Fe2+
-
Homo sapiens
2.4.1.214 GDP strong product inhibition, complete inhibition at 5 mM Homo sapiens
2.4.1.214 GMP strong inhibition Homo sapiens
2.4.1.214 GTP strong inhibition Homo sapiens
2.4.1.214 additional information no inhibition of the recombinant soluble enzyme by NEM Homo sapiens
2.4.1.214 NaCl 50% inhibition at 250 mM, complete inhibition at 500 mM and above Homo sapiens
2.4.1.214 UDP slight inhibition at 3-5 mM Homo sapiens
2.4.1.214 Zn2+
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.214 0.0062
-
GDP-alpha-L-fucose recombinant soluble enzyme Homo sapiens
2.4.1.214 0.5
-
fetuin recombinant soluble enzyme Homo sapiens
2.4.1.214 5.6
-
NeuAcalpha2-3Galbeta1-4GlcNAc recombinant soluble enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.214 Golgi apparatus lumenal side of the membrane Homo sapiens 5794
-
2.4.1.214 membrane
-
Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.214 Ca2+ less effective than Mn2+ Homo sapiens
2.4.1.214 Co2+ less effective than Mn2+ Homo sapiens
2.4.1.214 Mg2+ less effective than Mn2+ Homo sapiens
2.4.1.214 Mn2+ activates best at 15 mM Homo sapiens
2.4.1.214 additional information activation by divalent cations in descending order: Mn2+, Mg2+, Ca2+, Co2+, while Ni2+ has no effect on the enzyme activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.214 49000
-
x * 49000, recombinant enzyme glycoform 1, SDS-PAGE, x * 55000, recombinant enzyme glycoform 1, SDS-PAGE Homo sapiens
2.4.1.214 55000
-
x * 49000, recombinant enzyme glycoform 1, SDS-PAGE, x * 55000, recombinant enzyme glycoform 1, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.214 GDP-alpha-L-fucose + Galbeta1-4GlcNAc-R Homo sapiens
-
GDP + Galbeta1-4[Fucalpha1-3]GlcNAc-R
-
ir
2.4.1.214 additional information Homo sapiens the enzyme synthesizes functional selectin ligands on the surface of leukocytes ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.214 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.214 glycoprotein the recombinant enzyme exists in 2 glycoprotein forms of 55 and 49 kDa, cleaving by PNGaseF Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.214 recombinant soluble enzyme from CHO cells by GDP-hexanolamine affinity chromatography to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.214 leukocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.214 1.6
-
purified recombinant soluble enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.214 GDP-alpha-L-fucose + fetuin
-
Homo sapiens GDP + 3-alpha-L-fucosyl-fetuin
-
?
2.4.1.214 GDP-alpha-L-fucose + Galbeta1-4GlcNAc-R
-
Homo sapiens GDP + Galbeta1-4[Fucalpha1-3]GlcNAc-R
-
ir
2.4.1.214 GDP-alpha-L-fucose + NeuAcalpha2-3Galbeta1-3GlcNAcbeta-R 4% of the activity with NeuAcalpha2-3Galbeta1-4GlcNAc-R Homo sapiens GDP + NeuAcalpha2-3Galbeta1-3[Fucalpha1-3]GlcNAcbeta-R
-
?
2.4.1.214 GDP-alpha-L-fucose + NeuAcalpha2-3Galbeta1-4GlcNAc 28% of the activity with NeuAcalpha2-3Galbeta1-4GlcNAc-R Homo sapiens GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAc
-
?
2.4.1.214 GDP-alpha-L-fucose + NeuAcalpha2-3Galbeta1-4GlcNAcbeta-R best substrate Homo sapiens GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAcbeta-R
-
?
2.4.1.214 additional information the enzyme synthesizes functional selectin ligands on the surface of leukocytes Homo sapiens ?
-
?
2.4.1.214 additional information poorly active with Galbeta1-4GlcNAc-O-(CH2)8-COOCH3, Fucalpha1-2Galbeta1-4GlcNAcbeta-R, Galbeta1-3GlcNAcbeta-R, and Fucalpha1-2Galbeta1-3GlcNAcbeta-R Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.214 ? x * 49000, recombinant enzyme glycoform 1, SDS-PAGE, x * 55000, recombinant enzyme glycoform 1, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.214 alpha3-fucosyltransferase
-
Homo sapiens
2.4.1.214 FucT VII
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.214 7 10 the recombinant enzyme shows a broad pH-optimum Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.214 6 11 enzyme is not affected by high pH but shows no activity at pH below pH 5.5 Homo sapiens