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Literature summary extracted from

  • Nakao, H.; Takeuchi, K.; Shinoda, S.; Yamamoto, S.
    L-2,4,-Diaminobutyric acid decarboxylase activity responsible for the formation of 1,3-diaminopropane in Enterobacter aerogenes (1990), FEMS Microbiol. Lett., 70, 61-66.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.86 DTT activates 8fold at 5-7 mM Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.86 carboxymethoxylamine PLP-dependent inhibitor, 42% inhibition at 0.5 mM, 65% inhibition at 1 mM Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.86 0.32
-
 L-2,4-diaminobutanoate pH 8.3, 37°C Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.86 Ca2+ activates 14fold at 20 mM Klebsiella aerogenes
4.1.1.86 Cu2+ activates 7fold at 20 mM Klebsiella aerogenes
4.1.1.86 Mg2+ required, activates 20fold at 20 mM, enhances activation by PLP Klebsiella aerogenes
4.1.1.86 Mn2+ activates 5fold at 20 mM Klebsiella aerogenes
4.1.1.86 Zn2+ activates 3fold at 20 mM Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate Klebsiella aerogenes the enzyme is involved in polyamine biosynthesis propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Klebsiella aerogenes NCTC 10006 the enzyme is involved in polyamine biosynthesis propane-1,3-diamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.86 Klebsiella aerogenes
-
-
-
4.1.1.86 Klebsiella aerogenes NCTC 10006
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.86 native enzyme 360fold Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.86 1.2
-
 partially purified enzyme Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate
-
 Klebsiella aerogenes propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate the enzyme is involved in polyamine biosynthesis Klebsiella aerogenes propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Klebsiella aerogenes NCTC 10006 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate the enzyme is involved in polyamine biosynthesis Klebsiella aerogenes NCTC 10006 propane-1,3-diamine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.86 DABA-DC
-
 Klebsiella aerogenes
4.1.1.86 L-2,4-diaminobutyric acid decarboxylase
-
 Klebsiella aerogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.86 37
-
 assay at Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.86 7.8 8
-
 Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.86 6 9 43% of maximal activity at pH 7.3, 60% at pH 9.0 Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.86 pyridoxal 5'-phosphate absolutely required for activity, highly activating in pesence of 20 mM Mg2+ Klebsiella aerogenes