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Literature summary extracted from

  • Klein, M.; Reichelt, M.; Gershenzon, J.; Papenbrock, J.
    The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed (2006), FEBS J., 273, 122-136.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.24 G301D isoenzyme AtSOT18 C24, 25times higher specific activity with desulfobenzylglucosinolate Arabidopsis thaliana
2.8.2.24 N339K to date no purification of the protein under native conditions Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.24 3'-phosphoadenosine-5'-phosphate
-
Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.24 additional information
-
additional information not detectable: 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT18 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 0.025
-
desulfobenzylglucosinolate isoenzyme AtSOT17 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 0.035
-
desulfobenzylglucosinolate isoenzyme AtSOT18 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 0.05
-
3'-phosphoadenosine-5'-phosphosulfate isoenzyme AtSOT18 Co10, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 0.06
-
desulfobenzylglucosinolate isoenzyme AtSOT18 Co10, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 0.1
-
3'-phosphoadenosine-5'-phosphosulfate isoenzyme AtSOT16 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 0.1
-
desulfobenzylglucosinolate isoenzyme AtSOT16 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 0.1
-
3'-phosphoadenosine-5'-phosphosulfate isoenzyme AtSOT17 C24, pH 9.0, 37°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.24 cytoplasm
-
Arabidopsis thaliana 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.24 39200
-
ecotype Co10, isoenzyme AtSOT16, calculated Arabidopsis thaliana
2.8.2.24 39900
-
ecotype Co10, isoenzyme AtSOT17, calculated Arabidopsis thaliana
2.8.2.24 40500
-
ecotype Co10, isoenzyme AtSOT18, calculated Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.24 Arabidopsis thaliana
-
ecotype C24, ecotype Co10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.24
-
Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.24 additional information
-
0.0016197 Vmax desulfobenzylglucosinolate, isoenzyme AtSOT18 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 additional information
-
0.007499 Vmax 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT17 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 additional information
-
0.008518 Vmax desulfobenzylglucosinolate, isoenzyme AtSOT17 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 additional information
-
0.01026 Vmax 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT18 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 additional information
-
0.0415 Vmax desulfobenzylglucosinolate, isoenzyme AtSOT16 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 additional information
-
0.05105 Vmax 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT18 Co10, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 additional information
-
0.05159 Vmax desulfobenzylglucosinolate, isoenzyme AtSOT18 Co10, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 additional information
-
0.06599 Vmax 3'-phosphoadenosine-5'-phosphosulfate, isoenzyme AtSOT16 C24, pH 9.0, 37°C Arabidopsis thaliana
2.8.2.24 additional information
-
isoenzyme AtSOT16 C24 best substrate: 2-phenylethyl-glucosinolate Arabidopsis thaliana
2.8.2.24 additional information
-
isoenzyme AtSOT17 C24 best substrate: benzylglucosinolate Arabidopsis thaliana
2.8.2.24 additional information
-
isoenzyme AtSOT18 C24 best substrate: 6-methylthiohexylglucosinolate Arabidopsis thaliana
2.8.2.24 additional information
-
isoenzyme AtSOT18 Co10 best substrate: benzylglucosinolate Arabidopsis thaliana
2.8.2.24 additional information
-
isoenzyme AtSOT18G301D best substrate: 4-methylthiobutylglucosinolate Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.24 3'-phosphoadenosine-5'-phosphosulfate + desulfobenzylglucosinolate
-
Arabidopsis thaliana adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.24 37
-
-
Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.24 9
-
-
Arabidopsis thaliana