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Literature summary extracted from
- Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl (2005), FEBS J., 272, 966-974.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.80 | expression in Escherichia coli | Paraburkholderia xenovorans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.80 | 0.0317 | - |
2-hydroxypent-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans |  |
| 4.2.1.80 | 0.0386 | - |
2-hydroxypent-2,4-dienoate | activated by Mn2+ | Paraburkholderia xenovorans | |
| 4.2.1.80 | 0.0408 | - |
2-hydroxyhexa-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
| 4.2.1.80 | 0.0459 | - |
5-chloro-2-hydroxypent-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.80 | Ca2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
| 4.2.1.80 | Co2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
| 4.2.1.80 | Mg2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
| 4.2.1.80 | Mn2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
| 4.2.1.80 | Zn2+ | divalent metal required, activation in order of decreasing efficiency: Mg2+, Mn2+, Co2+, Zn2+, Ca2+. Divalent metal ion is hexa-coordinated in the enzyme. It has a catalytic rather than just a substrate binding role. Enzyme has no activity when divalent cations are excluded from the assay mixture | Paraburkholderia xenovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.80 | 27000 | - |
8 * 27000, SDS-PAGE | Paraburkholderia xenovorans |
| 4.2.1.80 | 221000 | - |
gel filtration | Paraburkholderia xenovorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.80 | 2-hydroxypent-2,4-dienoate + H2O | Paraburkholderia xenovorans | reaction in the catabolic pathway of numerous aromatic compounds, including polychlorinated biphenyls | 2-keto-4-hydroxypentanoate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.80 | Paraburkholderia xenovorans | - |
LB400 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.80 | recombinant | Paraburkholderia xenovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.80 | 2-hydroxyhexa-2,4-dienoate + H2O | - |
Paraburkholderia xenovorans | 2-keto-4-hydroxyhexanoate | - |
? | |
| 4.2.1.80 | 2-hydroxypent-2,4-dienoate + H2O | - |
Paraburkholderia xenovorans | 2-keto-4-hydroxypentanoate | - |
? | |
| 4.2.1.80 | 2-hydroxypent-2,4-dienoate + H2O | reaction in the catabolic pathway of numerous aromatic compounds, including polychlorinated biphenyls | Paraburkholderia xenovorans | 2-keto-4-hydroxypentanoate | - |
? | |
| 4.2.1.80 | 5-chloro-2-hydroxypent-2,4-dienoate | - |
Paraburkholderia xenovorans | 5-chloro-2-keto-4-hydroxypentanoate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.80 | octamer | 8 * 27000, SDS-PAGE | Paraburkholderia xenovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.80 | BphH | - |
Paraburkholderia xenovorans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.80 | 7.7 | - |
5-chloro-2-hydroxypent-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
| 4.2.1.80 | 60.5 | - |
2-hydroxyhexa-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
| 4.2.1.80 | 215 | - |
2-hydroxypent-2,4-dienoate | activated by Mn2+ | Paraburkholderia xenovorans | |
| 4.2.1.80 | 361 | - |
2-hydroxypent-2,4-dienoate | activated by Mg2+ | Paraburkholderia xenovorans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.80 | 6 | 8 | - |
Paraburkholderia xenovorans |
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