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Literature summary extracted from

  • Vatter, P.; Stoesser, C.; Samel, I.; Gierschik, P.; Moepps, B.
    The variable C-terminal extension of G-protein-coupled receptor kinase 6 constitutes an accessorial autoregulatory domain (2005), FEBS J., 272, 6039-6051.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.16 expression of isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D in Spodoptera frugiperda Sf9 cells via baculovirus infection system, transient expression of isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D in COS-7 cells, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C are membrane-associated, recombinant isoform mGRK6-D is inactive and located in the nucleus Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.16 additional information construction of isozymes mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D by alternative splicing, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C are membrane-associated, recombinant isoform mGRK6-D is inactive and located in the nucleus, structure-activity relationships of the enzyme C-terminus, overview Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.16 additional information the variable C-terminal extension of GRK6 constitutes a domain with autoinhibitory function involving residues D560, S566, and L576 of 3 inhibitory elements and an intervening stimulatory element Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.16 Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.16 ATP + [G-protein-coupled receptor] Mus musculus
-
 ADP + [G-protein-coupled receptor]phosphate
-
 ?
2.7.11.16 additional information Mus musculus the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.16 Mus musculus
-
 4 isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D by alternative splicing
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.16 lipoprotein the C-terminus of GRK6 is palmitoylated, not required for phosphorylation activity Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.16 recombinant isozymes mGRK6-A, mGRK6-B, and mGRK-C from Sf9 insect cells by sequential gel filtration and heparin affinity chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.16 ATP + rhodopsin light-activated rhodopsin, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C, the latter showing highest activity Mus musculus ADP + phosphorylated rhodopsin
-
 ?
2.7.11.16 ATP + [G-protein-coupled receptor]
-
 Mus musculus ADP + [G-protein-coupled receptor]phosphate
-
 ?
2.7.11.16 additional information the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain Mus musculus ?
-
 ?
2.7.11.16 additional information structure-activity relationships of the enzyme C-terminus, overview Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.16 More the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain consisting of 3 autoregulatory elements involving residues D560, S566, and L576 and an intervening stimulatory element Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.16 G-protein-coupled receptor kinase 6
-
 Mus musculus
2.7.11.16 GRK6
-
 Mus musculus
2.7.11.16 More GRK6 belongs to the GRK4 subfamily of the GRK family Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.16 30
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.16 7.5
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.16 ATP
-
 Mus musculus