EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.2 | wild-type and L109A recombinant enzyme are expressed in Escherichia coli BL21(DE3) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.2 | L109A | uncoupling of the hydrolysis of gamma-glutamyl hydroxamate and nascent NH2OH production from N4-hydroxy-CTP formation is more pronounced with mutant than with wild-type enzyme | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.2 | 0.063 | - |
Gln-OH | 37°C, pH 8.0, mutant enzyme L109A | Escherichia coli | |
6.3.4.2 | 0.165 | - |
Gln-OH | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 0.354 | - |
Gln | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 0.497 | - |
Gln | 37°C, pH 8.0, mutant enzyme L109A | Escherichia coli | |
6.3.4.2 | 2.15 | - |
NH3 | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 2.17 | - |
NH3 | 37°C, pH 8.0, mutant enzyme L109A | Escherichia coli | |
6.3.4.2 | 39.4 | - |
L-glutamine | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 75.3 | - |
NH2OH | 37°C, pH 8.0, mutant enzyme L109A | Escherichia coli | |
6.3.4.2 | 82.8 | - |
NH2OH | 37°C, pH 8.0, wild-type enzyme | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.2 | Escherichia coli | P0A7E5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.2 | recombinant wild-type and mutant enzyme L109A | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.2 | ATP + UTP + gamma-L-glutamylhydroxamate | - |
Escherichia coli | ADP + phosphate + N4-hydroxy-CTP | - |
? | |
6.3.4.2 | ATP + UTP + Gln | - |
Escherichia coli | ADP + phosphate + CTP + Glu | - |
? | |
6.3.4.2 | ATP + UTP + L-gamma-glutamyl hydrazide | - |
Escherichia coli | ADP + phosphate + N4-amino-CTP + L-glutamate | - |
? | |
6.3.4.2 | ATP + UTP + NH2NH2 | - |
Escherichia coli | ADP + phosphate + CTP + N4-amino CTP | - |
? | |
6.3.4.2 | ATP + UTP + NH2OH | - |
Escherichia coli | ADP + phosphate + N4-hydroxy-CTP | - |
? | |
6.3.4.2 | ATP + UTP + NH4+ | - |
Escherichia coli | ADP + phosphate + CTP | - |
? | |
6.3.4.2 | L-glutamine + H2O | - |
Escherichia coli | L-glutamate + NH3 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.2 | 0.063 | - |
Gln-OH | 37°C, pH 8.0, mutant enzyme L109A | Escherichia coli | |
6.3.4.2 | 0.453 | - |
Gln-OH | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 1.41 | - |
L-glutamine | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 1.86 | - |
Gln | 37°C, pH 8.0, mutant enzyme L109A | Escherichia coli | |
6.3.4.2 | 6.1 | - |
Gln | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 9.5 | - |
NH3 | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 10.1 | - |
NH3 | 37°C, pH 8.0, mutant enzyme L109A | Escherichia coli | |
6.3.4.2 | 14 | - |
NH2OH | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
6.3.4.2 | 14.1 | - |
NH2OH | 37°C, pH 8.0, mutant enzyme L109A | Escherichia coli |