EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.11.16 | transient overexpression of wild-types and dominant negative mutants of GRK5 and GRK6 in COS-7 cells | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.11.16 | K215R | inactive dominant negative mutants of GRK5 or GRK6, overexpression of the GRK5 mutant in myometrial cells does not influence beta-adrenergic receptor sensitivity, while overexpression of GRK6 mutant leads to a 70% increase in isoproterenol-stimulated beta-adrenergic signaling via the beta-adrenergic receptor | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | heparin | GRK inhibitor | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | Mg2+ | - |
Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.16 | additional information | Rattus norvegicus | the GRK4-6 family member enzymes mediate beta-adrenergic receptor desensitization, the beta-adrenergic receptor is stimulated by agonists such as isoproterenol, cholera toxin, or forskolin, and induces cAMP production | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.16 | Rattus norvegicus | - |
Sprague-Dawley rats | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.11.16 | myometrium | primary cells | Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.16 | ATP + rhodopsin | urea treated outer segments of rhodopsin | Rattus norvegicus | ADP + phosphorylated rhodopsin | - |
? | |
2.7.11.16 | additional information | the GRK4-6 family member enzymes mediate beta-adrenergic receptor desensitization, the beta-adrenergic receptor is stimulated by agonists such as isoproterenol, cholera toxin, or forskolin, and induces cAMP production | Rattus norvegicus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.11.16 | G protein-coupled receptor kinase 5 | - |
Rattus norvegicus |
2.7.11.16 | G protein-coupled receptor kinase 6 | - |
Rattus norvegicus |
2.7.11.16 | GRK5 | - |
Rattus norvegicus |
2.7.11.16 | GRK6 | - |
Rattus norvegicus |
2.7.11.16 | More | the enzyme belongs to the GRK4-6 family | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.16 | 30 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.16 | 7.5 | - |
assay at | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | ATP | - |
Rattus norvegicus |