Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Knecht, W.; Sandrini, M.P.; Johansson, K.; Eklund, H.; Munch-Petersen, B.; Piskur, J.
    A few amino acid substitutions can convert deoxyribonucleoside kinase specificity from pyrimidines to purines (2002), Embo J., 21, 1873-1880.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 expression of wild-type and mutant enzymes in Escherichia coli strain BL21 as C-terminally His8-tagged proteins Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.145 A110D site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 M88R site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 M88R/A110D site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 V84A site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 V84A/M88R/A110D site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 V84M site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 V84S site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 V84S/M88R/A110D site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 0.0012
-
thymidine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 0.0023
-
2'-deoxycytidine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 0.0025
-
2'-deoxycytidine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 0.0113
-
thymidine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 0.0129
-
2'-deoxycytidine recombinant mutant V84S Drosophila melanogaster
2.7.1.145 0.0247
-
thymidine recombinant mutant V84S Drosophila melanogaster
2.7.1.145 0.04
-
2'-deoxyguanosine recombinant mutant V84A/M88R/A110D Drosophila melanogaster
2.7.1.145 0.064
-
thymidine recombinant mutant A110D Drosophila melanogaster
2.7.1.145 0.091
-
2'-deoxycytidine recombinant mutant A110D Drosophila melanogaster
2.7.1.145 0.147
-
2'-deoxyguanosine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 0.15
-
2'-deoxyadenosine recombinant mutant V84S Drosophila melanogaster
2.7.1.145 0.153
-
2'-deoxyadenosine recombinant mutant V84A/M88R/A110D Drosophila melanogaster
2.7.1.145 0.159
-
2'-deoxyguanosine recombinant mutant V84S/M88R/A110D Drosophila melanogaster
2.7.1.145 0.225
-
2'-deoxyadenosine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 0.237
-
thymidine recombinant mutant M88R Drosophila melanogaster
2.7.1.145 0.239
-
2'-deoxyadenosine recombinant mutant A110D Drosophila melanogaster
2.7.1.145 0.257
-
2'-deoxyguanosine recombinant mutant A110D Drosophila melanogaster
2.7.1.145 0.292
-
Dideoxycytidine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 0.36
-
2'-deoxyadenosine recombinant mutant M88R/A110D Drosophila melanogaster
2.7.1.145 0.375
-
2'-deoxyguanosine recombinant mutant V84S Drosophila melanogaster
2.7.1.145 0.388
-
2'-deoxyadenosine recombinant mutant V84S/M88R/A110D Drosophila melanogaster
2.7.1.145 0.549
-
2'-deoxyguanosine recombinant mutant M88R/A110D Drosophila melanogaster
2.7.1.145 0.584
-
2'-deoxycytidine recombinant mutant M88R/A110D Drosophila melanogaster
2.7.1.145 0.627
-
2'-deoxycytidine recombinant mutant V84A/M88R/A110D Drosophila melanogaster
2.7.1.145 0.645
-
2'-deoxyguanosine recombinant mutant M88R Drosophila melanogaster
2.7.1.145 0.665
-
2'-deoxyguanosine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 0.674
-
2'-deoxycytidine recombinant mutant V84M Drosophila melanogaster
2.7.1.145 0.7
-
2'-deoxyadenosine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 0.829
-
thymidine recombinant mutant V84M Drosophila melanogaster
2.7.1.145 0.852
-
2'-deoxyadenosine recombinant mutant V84M Drosophila melanogaster
2.7.1.145 1.039
-
2'-deoxycytidine recombinant mutant M88R Drosophila melanogaster
2.7.1.145 1.124
-
Dideoxycytidine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 1.512
-
thymidine recombinant mutant V84A/M88R/A110D Drosophila melanogaster
2.7.1.145 1.911
-
2'-deoxyguanosine recombinant mutant V84M Drosophila melanogaster
2.7.1.145 2.088
-
2'-deoxyadenosine recombinant mutant M88R Drosophila melanogaster
2.7.1.145 2.162
-
2'-deoxycytidine recombinant mutant V84S/M88R/A110D Drosophila melanogaster
2.7.1.145 2.425
-
thymidine recombinant mutant M88R/A110D Drosophila melanogaster
2.7.1.145 6.56
-
thymidine recombinant mutant V84S/M88R/A110D Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.145 Mg2+
-
Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.145 additional information Drosophila melanogaster enzyme evolution by gene duplication of a progenitor kinase ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.145 recombinant C-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.145 additional information
-
catalytic efficiencies of wild-type and mutant enzymes, overview Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 2'-deoxyadenosine
-
Drosophila melanogaster ADP + 2'-deoxyadenosine 5'-phosphate
-
?
2.7.1.145 ATP + 2'-deoxycytidine wild-type enzyme: second-best substrate Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
?
2.7.1.145 ATP + 2'-deoxyguanosine
-
Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
?
2.7.1.145 ATP + dideoxycytidine wild-type enzyme: low activity Drosophila melanogaster ADP + dideoxycytidine 5'-phosphate
-
?
2.7.1.145 ATP + thymidine wild-type enzyme: preferred substrate Drosophila melanogaster ADP + thymidine 5'-phosphate
-
?
2.7.1.145 additional information enzyme evolution by gene duplication of a progenitor kinase Drosophila melanogaster ?
-
?
2.7.1.145 additional information amino acid residues responsible for substrate specificity are V84, M88, and A110, wild-type enzyme shows broad substrate specificity preferring pyrimidines to purines, substrate specificities of wild-type and mutant enzymes, overview Drosophila melanogaster ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 dNK
-
Drosophila melanogaster
2.7.1.145 multisubstrate deoxyribonucleoside kinase
-
Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.145 0.034
-
thymidine recombinant mutant M88R/A110D Drosophila melanogaster
2.7.1.145 0.037
-
thymidine recombinant mutant V84M Drosophila melanogaster
2.7.1.145 0.073
-
2'-deoxyguanosine recombinant mutant A110D Drosophila melanogaster
2.7.1.145 0.084
-
2'-deoxyadenosine recombinant mutant A110D Drosophila melanogaster
2.7.1.145 0.11
-
thymidine recombinant mutant V84A/M88R/A110D Drosophila melanogaster
2.7.1.145 0.13
-
thymidine recombinant mutant M88R Drosophila melanogaster
2.7.1.145 0.22
-
2'-deoxyguanosine recombinant mutant V84M Drosophila melanogaster
2.7.1.145 0.23
-
thymidine recombinant mutant V84S/M88R/A110D Drosophila melanogaster
2.7.1.145 0.27
-
2'-deoxycytidine recombinant mutant A110D Drosophila melanogaster
2.7.1.145 0.35
-
thymidine recombinant mutant A110D Drosophila melanogaster
2.7.1.145 0.64
-
2'-deoxyguanosine recombinant mutant V84S Drosophila melanogaster
2.7.1.145 0.83
-
thymidine recombinant mutant V84S Drosophila melanogaster
2.7.1.145 0.83
-
2'-deoxycytidine recombinant mutant V84S Drosophila melanogaster
2.7.1.145 0.93
-
Dideoxycytidine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 1.1
-
2'-deoxyadenosine recombinant mutant V84S Drosophila melanogaster
2.7.1.145 1.36
-
2'-deoxyadenosine recombinant mutant V84M Drosophila melanogaster
2.7.1.145 1.62
-
2'-deoxycytidine recombinant mutant V84S/M88R/A110D Drosophila melanogaster
2.7.1.145 1.73
-
2'-deoxyguanosine recombinant mutant V84A/M88R/A110D Drosophila melanogaster
2.7.1.145 1.8
-
2'-deoxycytidine recombinant mutant V84M Drosophila melanogaster
2.7.1.145 1.88
-
2'-deoxyadenosine recombinant mutant M88R Drosophila melanogaster
2.7.1.145 1.94
-
2'-deoxycytidine recombinant mutant V84A/M88R/A110D Drosophila melanogaster
2.7.1.145 2.7
-
2'-deoxyguanosine recombinant mutant M88R Drosophila melanogaster
2.7.1.145 2.8
-
2'-deoxyguanosine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 2.97
-
thymidine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 3.2
-
2'-deoxyadenosine recombinant mutant V84S/M88R/A110D Drosophila melanogaster
2.7.1.145 3.5
-
2'-deoxycytidine recombinant mutant M88R/A110D Drosophila melanogaster
2.7.1.145 3.5
-
2'-deoxyadenosine recombinant mutant V84A/M88R/A110D Drosophila melanogaster
2.7.1.145 3.7
-
2'-deoxyguanosine recombinant mutant M88R/A110D Drosophila melanogaster
2.7.1.145 3.7
-
2'-deoxyadenosine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 4.2
-
Dideoxycytidine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 4.4
-
2'-deoxycytidine recombinant mutant V84A Drosophila melanogaster
2.7.1.145 4.6
-
2'-deoxyguanosine recombinant mutant V84S/M88R/A110D Drosophila melanogaster
2.7.1.145 6.9
-
2'-deoxyadenosine recombinant mutant M88R/A110D Drosophila melanogaster
2.7.1.145 7.5
-
2'-deoxycytidine recombinant mutant M88R Drosophila melanogaster
2.7.1.145 14.2
-
thymidine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 15.1
-
2'-deoxyguanosine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 16.5
-
2'-deoxycytidine recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 20.6
-
2'-deoxyadenosine recombinant wild-type enzyme Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.145 ATP
-
Drosophila melanogaster