EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.145 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21 as C-terminally His8-tagged proteins | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.145 | A110D | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | M88R | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | M88R/A110D | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | V84A | site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | V84A/M88R/A110D | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | V84M | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | V84S | site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | V84S/M88R/A110D | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.145 | 0.0012 | - |
thymidine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 0.0023 | - |
2'-deoxycytidine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 0.0025 | - |
2'-deoxycytidine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 0.0113 | - |
thymidine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 0.0129 | - |
2'-deoxycytidine | recombinant mutant V84S | Drosophila melanogaster | |
2.7.1.145 | 0.0247 | - |
thymidine | recombinant mutant V84S | Drosophila melanogaster | |
2.7.1.145 | 0.04 | - |
2'-deoxyguanosine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.064 | - |
thymidine | recombinant mutant A110D | Drosophila melanogaster | |
2.7.1.145 | 0.091 | - |
2'-deoxycytidine | recombinant mutant A110D | Drosophila melanogaster | |
2.7.1.145 | 0.147 | - |
2'-deoxyguanosine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 0.15 | - |
2'-deoxyadenosine | recombinant mutant V84S | Drosophila melanogaster | |
2.7.1.145 | 0.153 | - |
2'-deoxyadenosine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.159 | - |
2'-deoxyguanosine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.225 | - |
2'-deoxyadenosine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 0.237 | - |
thymidine | recombinant mutant M88R | Drosophila melanogaster | |
2.7.1.145 | 0.239 | - |
2'-deoxyadenosine | recombinant mutant A110D | Drosophila melanogaster | |
2.7.1.145 | 0.257 | - |
2'-deoxyguanosine | recombinant mutant A110D | Drosophila melanogaster | |
2.7.1.145 | 0.292 | - |
Dideoxycytidine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 0.36 | - |
2'-deoxyadenosine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.375 | - |
2'-deoxyguanosine | recombinant mutant V84S | Drosophila melanogaster | |
2.7.1.145 | 0.388 | - |
2'-deoxyadenosine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.549 | - |
2'-deoxyguanosine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.584 | - |
2'-deoxycytidine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.627 | - |
2'-deoxycytidine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.645 | - |
2'-deoxyguanosine | recombinant mutant M88R | Drosophila melanogaster | |
2.7.1.145 | 0.665 | - |
2'-deoxyguanosine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 0.674 | - |
2'-deoxycytidine | recombinant mutant V84M | Drosophila melanogaster | |
2.7.1.145 | 0.7 | - |
2'-deoxyadenosine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 0.829 | - |
thymidine | recombinant mutant V84M | Drosophila melanogaster | |
2.7.1.145 | 0.852 | - |
2'-deoxyadenosine | recombinant mutant V84M | Drosophila melanogaster | |
2.7.1.145 | 1.039 | - |
2'-deoxycytidine | recombinant mutant M88R | Drosophila melanogaster | |
2.7.1.145 | 1.124 | - |
Dideoxycytidine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 1.512 | - |
thymidine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 1.911 | - |
2'-deoxyguanosine | recombinant mutant V84M | Drosophila melanogaster | |
2.7.1.145 | 2.088 | - |
2'-deoxyadenosine | recombinant mutant M88R | Drosophila melanogaster | |
2.7.1.145 | 2.162 | - |
2'-deoxycytidine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 2.425 | - |
thymidine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 6.56 | - |
thymidine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | Mg2+ | - |
Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | additional information | Drosophila melanogaster | enzyme evolution by gene duplication of a progenitor kinase | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.145 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.145 | recombinant C-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography | Drosophila melanogaster |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.145 | additional information | - |
catalytic efficiencies of wild-type and mutant enzymes, overview | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | ATP + 2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxycytidine | wild-type enzyme: second-best substrate | Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyguanosine | - |
Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + dideoxycytidine | wild-type enzyme: low activity | Drosophila melanogaster | ADP + dideoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + thymidine | wild-type enzyme: preferred substrate | Drosophila melanogaster | ADP + thymidine 5'-phosphate | - |
? | |
2.7.1.145 | additional information | enzyme evolution by gene duplication of a progenitor kinase | Drosophila melanogaster | ? | - |
? | |
2.7.1.145 | additional information | amino acid residues responsible for substrate specificity are V84, M88, and A110, wild-type enzyme shows broad substrate specificity preferring pyrimidines to purines, substrate specificities of wild-type and mutant enzymes, overview | Drosophila melanogaster | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.145 | dNK | - |
Drosophila melanogaster |
2.7.1.145 | multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.145 | 0.034 | - |
thymidine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.037 | - |
thymidine | recombinant mutant V84M | Drosophila melanogaster | |
2.7.1.145 | 0.073 | - |
2'-deoxyguanosine | recombinant mutant A110D | Drosophila melanogaster | |
2.7.1.145 | 0.084 | - |
2'-deoxyadenosine | recombinant mutant A110D | Drosophila melanogaster | |
2.7.1.145 | 0.11 | - |
thymidine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.13 | - |
thymidine | recombinant mutant M88R | Drosophila melanogaster | |
2.7.1.145 | 0.22 | - |
2'-deoxyguanosine | recombinant mutant V84M | Drosophila melanogaster | |
2.7.1.145 | 0.23 | - |
thymidine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 0.27 | - |
2'-deoxycytidine | recombinant mutant A110D | Drosophila melanogaster | |
2.7.1.145 | 0.35 | - |
thymidine | recombinant mutant A110D | Drosophila melanogaster | |
2.7.1.145 | 0.64 | - |
2'-deoxyguanosine | recombinant mutant V84S | Drosophila melanogaster | |
2.7.1.145 | 0.83 | - |
thymidine | recombinant mutant V84S | Drosophila melanogaster | |
2.7.1.145 | 0.83 | - |
2'-deoxycytidine | recombinant mutant V84S | Drosophila melanogaster | |
2.7.1.145 | 0.93 | - |
Dideoxycytidine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 1.1 | - |
2'-deoxyadenosine | recombinant mutant V84S | Drosophila melanogaster | |
2.7.1.145 | 1.36 | - |
2'-deoxyadenosine | recombinant mutant V84M | Drosophila melanogaster | |
2.7.1.145 | 1.62 | - |
2'-deoxycytidine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 1.73 | - |
2'-deoxyguanosine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 1.8 | - |
2'-deoxycytidine | recombinant mutant V84M | Drosophila melanogaster | |
2.7.1.145 | 1.88 | - |
2'-deoxyadenosine | recombinant mutant M88R | Drosophila melanogaster | |
2.7.1.145 | 1.94 | - |
2'-deoxycytidine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 2.7 | - |
2'-deoxyguanosine | recombinant mutant M88R | Drosophila melanogaster | |
2.7.1.145 | 2.8 | - |
2'-deoxyguanosine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 2.97 | - |
thymidine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 3.2 | - |
2'-deoxyadenosine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 3.5 | - |
2'-deoxycytidine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 3.5 | - |
2'-deoxyadenosine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 3.7 | - |
2'-deoxyguanosine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 3.7 | - |
2'-deoxyadenosine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 4.2 | - |
Dideoxycytidine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 4.4 | - |
2'-deoxycytidine | recombinant mutant V84A | Drosophila melanogaster | |
2.7.1.145 | 4.6 | - |
2'-deoxyguanosine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 6.9 | - |
2'-deoxyadenosine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
2.7.1.145 | 7.5 | - |
2'-deoxycytidine | recombinant mutant M88R | Drosophila melanogaster | |
2.7.1.145 | 14.2 | - |
thymidine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 15.1 | - |
2'-deoxyguanosine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 16.5 | - |
2'-deoxycytidine | recombinant wild-type enzyme | Drosophila melanogaster | |
2.7.1.145 | 20.6 | - |
2'-deoxyadenosine | recombinant wild-type enzyme | Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | ATP | - |
Drosophila melanogaster |