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Literature summary extracted from

  • Wada, M.; Awano, N.; Yamazawa, H.; Takagi, H.; Nakamori, S.
    Purification and characterization of O-acetylserine sulfhydrylase of Corynebacterium glutamicum (2004), Biosci. Biotechnol. Biochem., 68, 1581-1583.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 Cu2+
-
 Corynebacterium glutamicum
2.5.1.47 Hg2+
-
 Corynebacterium glutamicum
2.5.1.47 hydroxylamine
-
 Corynebacterium glutamicum
2.5.1.47 Ni2+
-
 Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.031
-
 Sulfide 37°C, pH 9.0 Corynebacterium glutamicum
2.5.1.47 7
-
 O3-acetyl-L-serine 37°C, pH 9.0 Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 34500
-
 2 * 34500, SDS-PAGE Corynebacterium glutamicum
2.5.1.47 70800
-
 gel filtration Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 435
-
 37°C, pH 9.0 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O3-acetyl-L-serine + sulfide
-
 Corynebacterium glutamicum L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 34500, SDS-PAGE Corynebacterium glutamicum
2.5.1.47 More N-terminal amino acid sequence Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 60
-
-
 Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47 45
-
 stable below Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.47 238
-
 O3-acetyl-L-serine 37°C, pH 9.0 Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 9
-
-
 Corynebacterium glutamicum