EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.49 | overexpression in Escherichia coli | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.49 | DL-propargylglycine | 89% inhibition at 1 mM | Thermus thermophilus | |
2.5.1.49 | hydroxylamine | 95% inhibition at 1 mM, 76% at 0.1 mM | Thermus thermophilus | |
2.5.1.49 | L-methionine | 68% inhibition at 1 mM | Thermus thermophilus | |
2.5.1.49 | phenylhydrazine | 95% inhibition at 10 mM, 12% at 1 mM | Thermus thermophilus | |
2.5.1.49 | Semicarbazide | 76% inhibition at 10 mM, 12% at 1 mM | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.49 | 2 | - |
O-acetyl-L-homoserine | at a fixed sulfide concentration of 1 mM | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.49 | 44000 | - |
SDS-PAGE | Thermus thermophilus |
2.5.1.49 | 44160 | - |
calculated from amino acid sequence | Thermus thermophilus |
2.5.1.49 | 190000 | - |
gel filtration | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.49 | Thermus thermophilus | Q76K51 | - |
- |
2.5.1.49 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q76K51 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.49 | - |
Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.49 | 3.17 | - |
crude extract | Thermus thermophilus |
2.5.1.49 | 14.3 | - |
after heat treatment at 65°C | Thermus thermophilus |
2.5.1.49 | 36 | - |
Q Sepharose pool | Thermus thermophilus |
2.5.1.49 | 67.7 | - |
phenyl Sepharose pool | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.49 | L-homoserine + H2S | lesser activity | Thermus thermophilus | L-homocysteine + H2O | - |
? | |
2.5.1.49 | L-homoserine + H2S | lesser activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-homocysteine + H2O | - |
? | |
2.5.1.49 | L-serine + H2S | lesser activity | Thermus thermophilus | L-cysteine + H2O | - |
? | |
2.5.1.49 | L-serine + H2S | lesser activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-cysteine + H2O | - |
? | |
2.5.1.49 | o-acetyl-L-homoserine + H2S | - |
Thermus thermophilus | L-homocysteine + acetate | - |
? | |
2.5.1.49 | o-acetyl-L-homoserine + H2S | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-homocysteine + acetate | - |
? | |
2.5.1.49 | O-acetyl-L-serine | lesser activity | Thermus thermophilus | L-cysteine + acetate | - |
? | |
2.5.1.49 | O-acetyl-L-serine | lesser activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-cysteine + acetate | - |
? | |
2.5.1.49 | O-phosphoryl-L-serine + H2S | lesser activity | Thermus thermophilus | L-cysteine + H2O | - |
? | |
2.5.1.49 | O-succinyl-L-homoserine + H2S | lesser activity | Thermus thermophilus | L-homocysteine + succinate | - |
? | |
2.5.1.49 | O-succinyl-L-homoserine + H2S | lesser activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-homocysteine + succinate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.49 | monomer | 1 * 44000, SDS-PAGE | Thermus thermophilus |
2.5.1.49 | tetramer | 4 * 44000, SDS-PAGE, gel filtration | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.49 | EC 4.2.99.10 | formerly | Thermus thermophilus |
2.5.1.49 | o-acetyl-L-homoserine sulfhydrylase | - |
Thermus thermophilus |
2.5.1.49 | OAH SHase | - |
Thermus thermophilus |
2.5.1.49 | oah2 gene product | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.49 | 70 | - |
- |
Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.49 | 90 | - |
80% activity remaining at 90°C for 60 min | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.49 | 7.5 | - |
- |
Thermus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.49 | 6.3 | 8.8 | less than 50% activity above and below | Thermus thermophilus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.49 | 7 | 12 | - |
Thermus thermophilus |