Literature summary extracted from
Yamamoto, S.; Mutoh, N.; Tsuzuki, D.; Ikai, H.; Nakao, H.; Shinoda, S.; Narimatsu, S.; Miyoshi, S.I.
Cloning and characterization of the ddc homolog encoding L-2,4-diaminobutyrate decarboxylase in Enterobacter aerogenes (2000), Biol. Pharm. Bull., 23, 649-653.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.1.1.86 |
gene ddc, DNA and amino acid sequence determination and analysis, restriction mapping, expression in Escherichia coli strain HB101 |
Klebsiella aerogenes |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.1.1.86 |
K198R |
site-directed mutagenesis, mutant shows 96% of the wild-type activity |
Klebsiella aerogenes |
4.1.1.86 |
K307R |
site-directed mutagenesis, mutant is catalytically inactive and shows slightly reduced molecular weight compared to the wild-type enzyme due to lacking pyridoxal 5'-phosphate |
Klebsiella aerogenes |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.1.1.86 |
53659 |
- |
x * 53659, DNA sequence calculation |
Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.1.1.86 |
L-2,4-diaminobutanoate |
Klebsiella aerogenes |
- |
propane-1,3-diamine + CO2 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.86 |
Klebsiella aerogenes |
Q9S0P8 |
gene ddc |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.86 |
L-2,4-diaminobutanoate |
- |
Klebsiella aerogenes |
propane-1,3-diamine + CO2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.1.1.86 |
? |
x * 53659, DNA sequence calculation |
Klebsiella aerogenes |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.1.86 |
DABA DC |
- |
Klebsiella aerogenes |
4.1.1.86 |
L-2,4-diaminobutyrate decarboxylase |
- |
Klebsiella aerogenes |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.1.1.86 |
pyridoxal 5'-phosphate |
binds to Lys307 |
Klebsiella aerogenes |
|