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Literature summary extracted from

  • Yamamoto, S.; Mutoh, N.; Tsuzuki, D.; Ikai, H.; Nakao, H.; Shinoda, S.; Narimatsu, S.; Miyoshi, S.I.
    Cloning and characterization of the ddc homolog encoding L-2,4-diaminobutyrate decarboxylase in Enterobacter aerogenes (2000), Biol. Pharm. Bull., 23, 649-653.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.86 gene ddc, DNA and amino acid sequence determination and analysis, restriction mapping, expression in Escherichia coli strain HB101 Klebsiella aerogenes

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.86 K198R site-directed mutagenesis, mutant shows 96% of the wild-type activity Klebsiella aerogenes
4.1.1.86 K307R site-directed mutagenesis, mutant is catalytically inactive and shows slightly reduced molecular weight compared to the wild-type enzyme due to lacking pyridoxal 5'-phosphate Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.86 53659
-
 x * 53659, DNA sequence calculation Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate Klebsiella aerogenes
-
 propane-1,3-diamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.86 Klebsiella aerogenes Q9S0P8 gene ddc
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate
-
 Klebsiella aerogenes propane-1,3-diamine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.86 ? x * 53659, DNA sequence calculation Klebsiella aerogenes

Synonyms

EC Number Synonyms Comment Organism
4.1.1.86 DABA DC
-
 Klebsiella aerogenes
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.86 pyridoxal 5'-phosphate binds to Lys307 Klebsiella aerogenes