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Literature summary extracted from

  • Iwersen, M.; Dora, H.; Kohla, G.; Gasa, S.; Schauer, R.
    Solubilisation and properties of the sialate-4-O-acetyltransferase from guinea pig liver (2003), Biol. Chem., 384, 1035-1047.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.44 3',5'-ADP 80% inhibition at 1 mM Cavia porcellus
2.3.1.44 3'-dephospho-coenzyme A 50% inhibition at 0.022 mM Cavia porcellus
2.3.1.44 ATP 20% inhibition at 1 mM Cavia porcellus
2.3.1.44 coenzyme A 50% inhibition at 0.013 mM Cavia porcellus
2.3.1.44 desulfo-coenzyme A 50% inhibition at 0.013 mM Cavia porcellus
2.3.1.44 IDP 30% inhibition at 1 mM Cavia porcellus
2.3.1.44 IMP 16% inhibition at 1 mM Cavia porcellus
2.3.1.44 ITP 58% inhibition at 1 mM Cavia porcellus
2.3.1.44 NADPH 18% inhibition at 1 mM Cavia porcellus
2.3.1.44 p-chloromercuribenzoate 100% inhibition at 1 mM Cavia porcellus
2.3.1.44 pyridoxal 5'-phosphate 75% inhibition at 1 mM Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.44 0.0012
-
 ganglioside GD3
-
 Cavia porcellus
2.3.1.44 0.0036
-
 acetyl-CoA
-
 Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.44 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.44 analysis of solubilisation by detergent CHAPS. Need for an unknown low molecular mass and heat-stable cofactor Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.44 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.44 additional information
-
 rapid method for measuring enzyme activity using ganglioside GD3 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.44 acetyl-CoA + ganglioside 9-O-acetyl-GD3 110% of the acitivity with ganglioside GD3 Cavia porcellus CoA + ganglioside 4,9-O-diacetyl-GD3
-
 ?
2.3.1.44 acetyl-CoA + ganglioside GD1a 90% of the acitivity with ganglioside GD3 Cavia porcellus CoA + 4-O-acetylganglioside GD1a
-
 ?
2.3.1.44 acetyl-CoA + ganglioside GD2 48% of the acitivity with ganglioside GD3 Cavia porcellus CoA + 4-O-acetylated ganglioside GD2
-
 ?
2.3.1.44 acetyl-CoA + ganglioside GD3
-
 Cavia porcellus CoA + 4-O-acetylated ganglioside GD3
-
 ?
2.3.1.44 acetyl-CoA + ganglioside GQ1b 125% of the acitivity with ganglioside GD3 Cavia porcellus CoA + 4-O-acetylated ganglioside GQ1b
-
 ?
2.3.1.44 acetyl-CoA + ganglioside GT1b 80% of the acitivity with ganglioside GD3 Cavia porcellus CoA + 4-O-acetylated ganglioside GT1b
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.44 30
-
 solubilized enzyme, presence of KCl and K2HPO4 Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.44 5.6
-
 solubilized enzyme, presence of KCl and K2HPO4 Cavia porcellus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.44 0.0148
-
 coenzyme A
-
 Cavia porcellus