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Literature summary extracted from

  • Pal, B.; Pybus, B.; Muccio, D.D.; Chattopadhyay, D.
    Biochemical characterization and crystallization of recombinant 3-phosphoglycerate kinase of Plasmodium falciparum (2004), Biochim. Biophys. Acta, 1699, 277-280.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.3 acetate as Na or K salt continues to activate at concentrations above 200 mM Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.3 expression in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 hanging-drop vapour-diffusion method, recombinant enzyme forms a hexagonal space group P6(1)22 with unit cell parameters a = b = 140.7 A, c = 263.9 A, 3.0 A resolution Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 KH2PO4 above 50 mM Plasmodium falciparum
2.7.2.3 NaH2PO4 above 50 mM Plasmodium falciparum
2.7.2.3 suramin IC50: 0.007 mM Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.52
-
 3-phospho-D-glycerate
-
 Plasmodium falciparum
2.7.2.3 0.63
-
 ATP
-
 Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 Ca2+ divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation Plasmodium falciparum
2.7.2.3 KCl maximal activation at 100 mM Plasmodium falciparum
2.7.2.3 KH2PO4 maximal activation at 25-50 mM Plasmodium falciparum
2.7.2.3 Mg2+ divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation. Optimal concentration of Mg2+ is 5-15 mM Plasmodium falciparum
2.7.2.3 Mn2+ divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation Plasmodium falciparum
2.7.2.3 NaCl maximal activation at 100 mM Plasmodium falciparum
2.7.2.3 NaH2PO4 maximal activation at 25-50 mM Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 45000
-
 x * 45000, SDS-PAGE Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 recombinant enzyme Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 210
-
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Plasmodium falciparum ADP + 3-phospho-D-glyceroyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.3 ? x * 45000, SDS-PAGE Plasmodium falciparum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 25
-
 30 min, 27% loss of activity Plasmodium falciparum
2.7.2.3 37
-
 30 min, nearly 80% loss of activity Plasmodium falciparum
2.7.2.3 49
-
 30 min, approximately 95% loss of activity Plasmodium falciparum
2.7.2.3 61
-
 30 min, enzyme retains less than 4% of its activity Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 7.5 7.9
-
 Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.2.3 0.007
-
 IC50: 0.007 mM Plasmodium falciparum suramin