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Literature summary extracted from

  • Reshetnikov, A.S.; Mustakhimov, II; Khmelenina, V.N.; Trotsenko, Y.A.
    Cloning, purification, and characterization of diaminobutyrate acetyltransferase from the halotolerant methanotroph Methylomicrobium alcaliphilum 20Z (2005), Biochemistry (Moscow), 70, 878-883.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.178 expression in Escherichia coli with an additional six His residues at the C-terminus Methylotuvimicrobium alcaliphilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.178 ATP 10 mM, 26% inhibition, 1 mM, 18% inhibition Methylotuvimicrobium alcaliphilum
2.3.1.178 Cd2+ 1 mM, complete inhibition Methylotuvimicrobium alcaliphilum
2.3.1.178 Zn2+ 1 mM, complete inhibition Methylotuvimicrobium alcaliphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.178 0.0366
-
acetyl-CoA
-
Methylotuvimicrobium alcaliphilum
2.3.1.178 0.46
-
L-2,4-diaminobutanoate
-
Methylotuvimicrobium alcaliphilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.178 KCl 0.25 M, activity increases threefold Methylotuvimicrobium alcaliphilum
2.3.1.178 NaCl 0.1-0.15 M, activity increases threefold Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.178 20000
-
2 * 20000, SDS-PAGE Methylotuvimicrobium alcaliphilum
2.3.1.178 40000
-
gel filtration Methylotuvimicrobium alcaliphilum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.178 Methylotuvimicrobium alcaliphilum
-
20Z
-
2.3.1.178 Methylotuvimicrobium alcaliphilum 20Z
-
20Z
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.178
-
Methylotuvimicrobium alcaliphilum

Storage Stability

EC Number Storage Stability Organism
2.3.1.178 4°C or -70°C, protein concentration 0.5 mg/ml, stable for at least 1 month in 50 mM Tris-HCl, pH 8.5, containing 0.2 M KCl Methylotuvimicrobium alcaliphilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
Methylotuvimicrobium alcaliphilum CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
Methylotuvimicrobium alcaliphilum 20Z CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?
2.3.1.178 propionyl-CoA + L-2,4-diaminobutanoate 20% of the activity with acetyl-CoA Methylotuvimicrobium alcaliphilum CoA + N4-propionyl-L-2,4-diaminobutanoate
-
?
2.3.1.178 propionyl-CoA + L-2,4-diaminobutanoate 20% of the activity with acetyl-CoA Methylotuvimicrobium alcaliphilum 20Z CoA + N4-propionyl-L-2,4-diaminobutanoate
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.178 dimer 2 * 20000, SDS-PAGE Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.178 20
-
-
Methylotuvimicrobium alcaliphilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.178 15 25 15°C: about 35% of maximal activity, 25°C: about 65% of maximal activity Methylotuvimicrobium alcaliphilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.178 9.5
-
-
Methylotuvimicrobium alcaliphilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.178 8 10.5 pH 8: about 50% of maximal activity, pH 10.5: about 70% of maximal activity Methylotuvimicrobium alcaliphilum