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Literature summary extracted from

  • Fogle, E.J.; Liu, W.; Woon, S.T.; Keller, J.W.; Toney, M.D.
    Role of Q52 in catalysis of decarboxylation and transamination in dialkylglycine decarboxylase (2005), Biochemistry, 44, 16392-16404.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.64 hanging drop vapor diffusion method, crystal structure of Q52A mutant and wild-type enzyme in complex with pyridoxamine 5'-phosphate Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.64 Q52A 85fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, greatly reduced rate of transamination half-reaction and impaired pyridoxamine phosphate binding Burkholderia cepacia
4.1.1.64 Q52E 10000fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, transamination rate is nearly identical to wild-type value Burkholderia cepacia
4.1.1.64 Q52I 100000fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, greatly reduced rate of transamination half-reaction and impaired pyridoxamine phosphate binding Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.64 0.015
-
 pyruvate pH 7.8, mutant enzyme Q52A Burkholderia cepacia
4.1.1.64 30
-
 2-aminoisobutanoate pH 7.8, mutant enzyme Q52A Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.64 Burkholderia cepacia P16932
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.64
-
 Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.64 2-Aminoisobutanoate + pyruvate
-
 Burkholderia cepacia L-Ala + acetone + CO2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.64 0.12
-
 pyruvate pH 7.8, mutant enzyme Q52A Burkholderia cepacia
4.1.1.64 0.12
-
 2-aminoisobutanoate pH 7.8, mutant enzyme Q52A Burkholderia cepacia