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Literature summary extracted from

  • Phannachet, K.; Elias, Y.; Huang, R.H.
    Dissecting the roles of a strictly conserved tyrosine in substrate recognition and catalysis by pseudouridine 55 synthase (2005), Biochemistry, 44, 15488-15494.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.25 crystal structure of the Y67F mutant in complex with a 5-fluorouridine-tRNA at 2.4 A resolution Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.25 Y67F no activity with the natural RNA substrate, efficient formation of 5-fluoro-6-hydroxypseudouridine55 from 5-fluorouridine55 Thermotoga maritima
5.4.99.25 Y67L no activity with the natural RNA substrate, efficient formation of 5-fluoro-6-hydroxypseudouridine55 from 5-fluorouridine55 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.25 0.00087
-
 tRNA uridine55 wild-type enzyme Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.25 Thermotoga maritima Q9WZW0
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.99.25 tRNA uridine55 = tRNA pseudouridine55 the side chain of the conserved tyrosine in the four families of pseudouridine synthases (TruA, TruB, RsuA and RluA) plays a dual role within the active site, maintaining the structural integrity of the active site through its hydrophobic phenyl ring and acting as a general base through its OH group for the proton abstraction required in the last step of pseudouridine synthase-catalyzed formation of pseudouridine Thermotoga maritima
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.25 additional information efficient formation of 5-fluoro-6-hydroxypseudouridine55 from 5-fluorouridine55 is catalyzed by wild-type enzyme and mutant enzymes Y67F and Y67L Thermotoga maritima ?
-
 ?
5.4.99.25 tRNA uridine55
-
 Thermotoga maritima tRNA pseudouridine55
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.25 pseudouridine 55 synthase
-
 Thermotoga maritima
5.4.99.25 psi55S
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.25 0.18
-
 tRNA uridine55 wild-type enzyme Thermotoga maritima