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Literature summary extracted from

  • Zheng, R.; Dam, T.K.; Brewer, C.F.; Blanchard, J.S.
    Active site residues in Mycobacterium tuberculosis pantothenate synthetase required in the formation and stabilization of the adenylate intermediate (2004), Biochemistry, 43, 7171-7178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.1 wild-type and mutant enzymes expressed in Escherichia coli BL21 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.1 H44A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis
6.3.2.1 H47A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis
6.3.2.1 K160A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis
6.3.2.1 K160C activity is markedly enhanced by the alkylation of cysteine with bromoethylamine Mycobacterium tuberculosis
6.3.2.1 N69A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis
6.3.2.1 Q164A mutant exhibits 50fold less activity than wild-type enzyme Mycobacterium tuberculosis
6.3.2.1 Q72A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.1 0.09
-
 beta-Alanine 25°C, pH 7.8, mutant enzyme K160C Mycobacterium tuberculosis
6.3.2.1 0.27
-
 beta-Alanine 25°C, pH 7.8, wild-type enzyme Mycobacterium tuberculosis
6.3.2.1 0.3
-
 beta-Alanine 25°C, pH 7.8, mutant enzyme Q164A Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.1
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
 Mycobacterium tuberculosis AMP + diphosphate + (R)-pantothenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.1 Pantothenate synthetase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.1 0.01
-
 beta-Alanine 25°C, pH 7.8, mutant enzyme K160C Mycobacterium tuberculosis
6.3.2.1 0.02
-
 beta-Alanine 25°C, pH 7.8, mutant enzyme Q164A Mycobacterium tuberculosis
6.3.2.1 1.01
-
 beta-Alanine 25°C, pH 7.8, wild-type enzyme Mycobacterium tuberculosis