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Literature summary extracted from

  • Miller, B.G.; Raines, R.T.
    Identifying latent enzyme activities: substrate ambiguity within modern bacterial sugar kinases (2004), Biochemistry, 43, 6387-6392.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.2 expression of genes nanK, yajF, and ycfX in glk-deficient mutant strain BM5340(DE3) Escherichia coli K-12

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.2 additional information construction of an enzyme-deficient glk mutant strain BM5340(DE3), the mutant strain can be complemented by expression of Escherichia coli genes nanK, yajF, and ycfX, which express proteins with 4fold lower glucokinase activity and ambiguous substrate specificities, overview Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 0.076
-
 D-glucose recombinant Glk, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 0.26
-
 ATP recombinant Glk, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 0.31
-
 D-glucose recombinant YajF, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 0.51
-
 ATP recombinant YajF, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 2.9
-
 ATP recombinant NanK, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 3.4
-
 ATP recombinant YcfX, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 3.8
-
 D-glucose recombinant YcfX, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 18
-
 D-glucose recombinant NanK, pH 7.6, 25°C Escherichia coli K-12

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.2 soluble
-
 Escherichia coli K-12
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 Mg2+
-
 Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.2 ATP + D-glucose Escherichia coli K-12
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Escherichia coli K-12
-
 several strains
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + D-glucose
-
 Escherichia coli K-12 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 additional information recombinant enzymes NanK and YajF also utilize N-acetyl-D-mannosamine and D-fructose as substrates, respectively Escherichia coli K-12 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.2 glk
-
 Escherichia coli K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.2 25
-
 assay at Escherichia coli K-12

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.2 9.1
-
 D-glucose recombinant NanK, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 9.2
-
 D-glucose recombinant YcfX, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 12
-
 D-glucose recombinant Glk, pH 7.6, 25°C Escherichia coli K-12
2.7.1.2 410
-
 D-glucose recombinant Glk, pH 7.6, 25°C Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 7.6
-
 assay at Escherichia coli K-12

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.2 ATP
-
 Escherichia coli K-12