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Literature summary extracted from

  • Yang, J.; Wang, Y.; Woolridge, E.M.; Arora, V.; Petsko, G.A.; Kozarich, J.W.; Ringe, D.
    Crystal structure of 3-carboxy-cis,cis-muconate lactonizing enzyme from Pseudomonas putida, a fumarase class II type cycloisomerase: Enzyme Evolution in Parallel Pathways (2004), Biochemistry, 43, 10424-10434.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.2 expressed in Escherichia coli strain N4830 Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.5.1.2 hanging drop vapour diffusion method with 12-18% PEG8000, 5 mM dithiothreitol, and 40 mM sodium phosphate (pH 7.3) Pseudomonas putida
5.5.1.2 hanging-drop vapor-diffusion method, 2.6 A resolution, symmetric of hexagonal space group P6(5) with unit cell parameters: a = b = 231.52 A, c = 78.46 A, alpha = beta = 90°, gamma = 120°. There are four PpCMLE molecules, forming a homotetramer, in the asymmetric unit Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.2 additional information requires no metal cofactor for catalytic activity Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.2
-
 Pseudomonas putida
5.5.1.2 DEAE-Sepharcel column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.2 3-carboxy-cis,cis-muconate
-
 Pseudomonas putida 4-carboxy-(S)-muconolactone
-
 r
5.5.1.2 additional information adenylosuccinate is not a substrate Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.2 homotetramer x-ray crystallography Pseudomonas putida
5.5.1.2 tetramer
-
 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
5.5.1.2 3-Carboxy-cis,cis-muconate lactonizing enzyme
-
 Pseudomonas putida
5.5.1.2 3-Carboxy-cis,cis-muconate lactonizing enzyme belongs to the fumarase class II superfamily Pseudomonas putida
5.5.1.2 CMLE
-
 Pseudomonas putida
5.5.1.2 PpCMLE
-
 Pseudomonas putida