Literature summary extracted from
Jang do, S.; Cha, H.J.; Cha, S.S.; Hong, B.H.; Ha, N.C.; Lee, J.Y.; Oh, B.H.; Lee, H.S.; Choi, K.Y.
Structural double-mutant cycle analysis of a hydrogen bond network in ketosteroid isomerase from Pseudomonas putida biotype B (2004), Biochem. J., 382, 967-973.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.3.3.1 |
mutant Y14F/D99L, increase in hydrophobic interaction while disrupting the hydrogen bond network, mutants Y30F/D99L and Y55F/D99L, disruption of hydrogen bond network |
Pseudomonas putida |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.3.3.1 |
Y14F/D99L |
partly additive effect of mutations for both, catalysis and stability, increase in hydrophobic interaction while disrupting the hydrogen bond network, crystallization data |
Pseudomonas putida |
5.3.3.1 |
Y30F/D99L |
disruption of hydrogen bond network, crystallization data |
Pseudomonas putida |
5.3.3.1 |
Y55F/D99L |
disruption of hydrogen bond network, crystallization data |
Pseudomonas putida |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.3.3.1 |
Pseudomonas putida |
- |
biotype B |
- |