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Literature summary extracted from

  • Li, C.L.; Lu, C.Y.; Ke, P.Y.; Chang, Z.F.
    Perturbation of ATP-induced tetramerization of human cytosolic thymidine kinase by substitution of serine-13 with aspartic acid at the mitotic phosphorylation site (2004), Biochem. Biophys. Res. Commun., 313, 587-593.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.21 ATP binding of ATP induces enzyme tetramerization, which leads to activation of the enzyme, the dimeric enzyme form is probably less active, 50-100% activation at up to 5 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B834(DE3), transient expression as Flag-tagged enzyme in LM-tk- cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.21 S13D site-directed mutagenesis, ATP-induced tetramerization is perturbed, mutant is less activated by ATP and shows reduced activity compared to the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 dTTP competitive feedback inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.00054
-
 thymidine pH 8.0, 37°C, recombinant wild-type enzyme Homo sapiens
2.7.1.21 0.00596
-
 thymidine pH 8.0, 37°C, recombinant mutant S13D Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 50000
-
 2 * 50000, the enzyme exists as a dimer and as a tetramer Homo sapiens
2.7.1.21 50000
-
 4 * 50000, the enzyme exists as a dimer and as a tetramer Homo sapiens
2.7.1.21 100000
-
 recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.21 ATP + 2'-deoxythymidine Homo sapiens
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + thymidine Homo sapiens
-
 ADP + thymidine 5'-phosphate
-
 ?
2.7.1.21 additional information Homo sapiens the enzyme is tightly regulated in the cell cycle by multiple mechanism, e.g. by phosphorylation at Ser13 in mitotic-arrested cells, regulation model ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens
-
 enzyme contains a Val at position 106
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.21 phosphoprotein phosphorylation at Ser13 has regulatory function Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain B834(DE3) by nickel affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2'-deoxythymidine
-
 Homo sapiens ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + thymidine
-
 Homo sapiens ADP + thymidine 5'-phosphate
-
 ?
2.7.1.21 additional information the enzyme is tightly regulated in the cell cycle by multiple mechanism, e.g. by phosphorylation at Ser13 in mitotic-arrested cells, regulation model Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 dimer 2 * 50000, the enzyme exists as a dimer and as a tetramer Homo sapiens
2.7.1.21 More binding of ATP induces enzyme tetramerization, which may lead to activation of the enzyme, the dimeric enzyme form is probably less active Homo sapiens
2.7.1.21 tetramer 4 * 50000, the enzyme exists as a dimer and as a tetramer Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 thymidine kinase
-
 Homo sapiens
2.7.1.21 TK1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.21 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.21 265.5
-
 thymidine pH 8.0, 37°C, recombinant mutant S13D Homo sapiens
2.7.1.21 282
-
 thymidine pH 8.0, 37°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 8
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.21 ATP binding of ATP induces enzyme tetramerization Homo sapiens