Literature summary extracted from
Zheng, X.; Johansson, M.; Karlsson, A.
Nucleoside analog cytotoxicity and bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in the nucleus or cytosol (2001), Biochem. Biophys. Res. Commun., 289, 229-233.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.7.1.145 |
medicine |
the enzyme can be used as a suicide gene in combined gene/chemotherapy treatment of cancer |
Drosophila melanogaster |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.1.145 |
overexpression of wild-type and mutant enzyme fused to GFP in cancer cell lines in the nucleus and cytosol, respectively |
Drosophila melanogaster |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.1.145 |
additional information |
overexpression in cancer cell lines leads to increased cell sensitivity to several cytotoxic nucleoside analogues, expression of wild-type and mutant enzyme in an osteosarcoma cell line utilizing a replication-deficient retroviral vector reveals that the localization of the enzyme in cytosol or nucleus is not important for cell sensitivity and bystander cell killing |
Drosophila melanogaster |
2.7.1.145 |
R247S |
site-directed mutagenesis, mutation abolishes nuclear import of the enzyme, the mutant enzyme is localized in the cytosol |
Drosophila melanogaster |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.1.145 |
additional information |
increased cell sensitivity to nucleoside analogues E-5-(2-bromovinyl)-2'-deoxyuridine and 1-beta-D-arabinofuranosylthymine in recombinant cancer cells overexpressing the enzyme in the nucleus or cytosol |
Drosophila melanogaster |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.7.1.145 |
nucleus |
- |
Drosophila melanogaster |
5634 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.1.145 |
60000 |
- |
x * 60000, about, recombinant wild-type and mutant GFP-fusion protein, SDS-PAGE |
Drosophila melanogaster |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.145 |
Drosophila melanogaster |
- |
- |
- |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.1.145 |
? |
x * 60000, about, recombinant wild-type and mutant GFP-fusion protein, SDS-PAGE |
Drosophila melanogaster |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.1.145 |
Dm-dNK |
- |
Drosophila melanogaster |