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Literature summary extracted from

  • Zheng, X.; Johansson, M.; Karlsson, A.
    Nucleoside analog cytotoxicity and bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in the nucleus or cytosol (2001), Biochem. Biophys. Res. Commun., 289, 229-233.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.145 medicine the enzyme can be used as a suicide gene in combined gene/chemotherapy treatment of cancer Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 overexpression of wild-type and mutant enzyme fused to GFP in cancer cell lines in the nucleus and cytosol, respectively Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.145 additional information overexpression in cancer cell lines leads to increased cell sensitivity to several cytotoxic nucleoside analogues, expression of wild-type and mutant enzyme in an osteosarcoma cell line utilizing a replication-deficient retroviral vector reveals that the localization of the enzyme in cytosol or nucleus is not important for cell sensitivity and bystander cell killing Drosophila melanogaster
2.7.1.145 R247S site-directed mutagenesis, mutation abolishes nuclear import of the enzyme, the mutant enzyme is localized in the cytosol Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.145 additional information increased cell sensitivity to nucleoside analogues E-5-(2-bromovinyl)-2'-deoxyuridine and 1-beta-D-arabinofuranosylthymine in recombinant cancer cells overexpressing the enzyme in the nucleus or cytosol Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.145 nucleus
-
Drosophila melanogaster 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.145 60000
-
x * 60000, about, recombinant wild-type and mutant GFP-fusion protein, SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster
-
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.145 ? x * 60000, about, recombinant wild-type and mutant GFP-fusion protein, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 Dm-dNK
-
Drosophila melanogaster