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Literature summary extracted from

  • Lee, D.W.; Choe, E.A.; Kim, S.B.; Eom, S.H.; Hong, Y.H.; Lee, S.J.; Lee, H.S.; Lee, D.Y.; Pyun, Y.R.
    Distinct metal dependence for catalytic and structural functions in the L-arabinose isomerases from the mesophilic Bacillus halodurans and the thermophilic Geobacillus stearothermophilus (2005), Arch. Biochem. Biophys., 434, 333-343.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli Geobacillus stearothermophilus
5.3.1.4 expression in Escherichia coli Halalkalibacterium halodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 36
-
 L-arabinose
-
 Halalkalibacterium halodurans
5.3.1.4 63
-
 L-arabinose
-
 Geobacillus stearothermophilus
5.3.1.4 117
-
 D-galactose
-
 Geobacillus stearothermophilus
5.3.1.4 167
-
 D-galactose
-
 Halalkalibacterium halodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ 60% activation compared to Mn2+ Geobacillus stearothermophilus
5.3.1.4 Mg2+ 50% activation compared to Mn2+ Geobacillus stearothermophilus
5.3.1.4 Mn2+ approximately 0.06 eqiuvalents of Mn2+/subunit Halalkalibacterium halodurans
5.3.1.4 Mn2+ strong activator, approximately 0.06 eqiuvalents of Mn2+/subunit Geobacillus stearothermophilus
5.3.1.4 additional information the conversion of D-galactose into D-tagatose is not dependent on divalent metal ions Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 56090
-
 monomer, calculated from amino acid sequence Geobacillus stearothermophilus
5.3.1.4 56320
-
 monomer, calculated from amino acid sequence Halalkalibacterium halodurans
5.3.1.4 57000
-
 monomer, SDS-PAGE Geobacillus stearothermophilus
5.3.1.4 57000
-
 monomer, SDS-PAGE Halalkalibacterium halodurans
5.3.1.4 112000
-
 native gradient gel electrophoresis, weak band (dimer) Geobacillus stearothermophilus
5.3.1.4 112000
-
 native gradient gel electrophoresis, weak band (dimer) Halalkalibacterium halodurans
5.3.1.4 224000
-
 native gradient gel electrophoresis Geobacillus stearothermophilus
5.3.1.4 224000
-
 native gradient gel electrophoresis Halalkalibacterium halodurans
5.3.1.4 225000
-
 gel filtration Geobacillus stearothermophilus
5.3.1.4 225000
-
 gel filtration Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 D-galactose Geobacillus stearothermophilus at elevated temperatures in the presence of divalent metal ions D-tagatose
-
 ?
5.3.1.4 D-galactose Halalkalibacterium halodurans at elevated temperatures, not dependent on divalent metal ions D-tagatose
-
 ?
5.3.1.4 L-arabinose Geobacillus stearothermophilus
-
 L-ribulose
-
 ?
5.3.1.4 L-arabinose Halalkalibacterium halodurans
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Geobacillus stearothermophilus
-
-
-
5.3.1.4 Halalkalibacterium halodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 recombinant enzyme, to homogeneity Geobacillus stearothermophilus
5.3.1.4 recombinant enzyme, to homogeneity Halalkalibacterium halodurans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 1.3
-
 with D-galactose as substrate Halalkalibacterium halodurans
5.3.1.4 9
-
 with D-galactose as substrate Geobacillus stearothermophilus
5.3.1.4 33.1
-
 with L-arabinose as substrate Halalkalibacterium halodurans
5.3.1.4 36.5
-
 with L-arabinose as substrate Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose at elevated temperatures in the presence of divalent metal ions Geobacillus stearothermophilus D-tagatose
-
 ?
5.3.1.4 D-galactose at elevated temperatures, not dependent on divalent metal ions Halalkalibacterium halodurans D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Geobacillus stearothermophilus L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Halalkalibacterium halodurans L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 tetramer 4 * 57000, SDS-PAGE, gel filtration Geobacillus stearothermophilus
5.3.1.4 tetramer 4 * 57000, SDS-PAGE, gel filtration Halalkalibacterium halodurans

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 AI
-
 Geobacillus stearothermophilus
5.3.1.4 AI
-
 Halalkalibacterium halodurans
5.3.1.4 L-arabinose isomerase
-
 Geobacillus stearothermophilus
5.3.1.4 L-arabinose isomerase
-
 Halalkalibacterium halodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 50
-
-
 Halalkalibacterium halodurans
5.3.1.4 70
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 additional information
-
 EDTA-treated AI has a lower melting temperature (72°C) than the holoenzyme (78°C) Geobacillus stearothermophilus
5.3.1.4 additional information
-
 Tm is 65-67°C, unaffected by the presence of metal ions Halalkalibacterium halodurans
5.3.1.4 70
-
 half life: 20 min Halalkalibacterium halodurans
5.3.1.4 80
-
 half life: 52 min Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.4 1.2
-
 D-galactose
-
 Halalkalibacterium halodurans
5.3.1.4 8.4
-
 D-galactose
-
 Geobacillus stearothermophilus
5.3.1.4 31
-
 L-arabinose
-
 Halalkalibacterium halodurans
5.3.1.4 34.1
-
 L-arabinose
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7 7.5
-
 Geobacillus stearothermophilus
5.3.1.4 8
-
 at 50°C and in the presence of 1 mM Mn2+ Halalkalibacterium halodurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.4 5.5 9
-
 Geobacillus stearothermophilus
5.3.1.4 6 10
-
 Halalkalibacterium halodurans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.4 Geobacillus stearothermophilus
-
-
 5.6
5.3.1.4 Halalkalibacterium halodurans
-
-
 5.8