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Literature summary extracted from

  • Pichersky, E.; Lewinsohn, E.; Croteau, R.
    Purification and characterization of S-linalool synthase, an enzyme involved in the production of floral scent in Clarkia breweri (1995), Arch. Biochem. Biophys., 316, 803-807.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.25 DTT required for stability Clarkia breweri

General Stability

EC Number General Stability Organism
4.2.3.25 DTT is required for enzyme stability, in absence of DTT the purified enzyme loses 95% of its activity within 2 h at 4°C Clarkia breweri
4.2.3.25 hexane and pentane stabilize the enzyme Clarkia breweri

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.25 additional information no effects by 0.1% w/v Triton X-100 Clarkia breweri
4.2.3.25 SDS leads to complete loss of activity at about 0.1% Clarkia breweri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.25 0.0009
-
geranyl diphosphate pH 7.4, 20°C Clarkia breweri
4.2.3.25 0.045
-
Mn2+ cofactor Mn2+, pH 7.4, 20°C Clarkia breweri
4.2.3.25 0.33
-
Mg2+ cofactor Mg2+, pH 7.4, 20°C Clarkia breweri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.25 Mg2+ half as effective as Mn2+ Clarkia breweri
4.2.3.25 Mn2+ absolutely required for activity, preferred divalent cation Clarkia breweri
4.2.3.25 additional information no activity with Cu2+, Zn2+, Ca2+, Co2+ Clarkia breweri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.25 73000
-
gel filtration Clarkia breweri
4.2.3.25 79000
-
1 * 79000, SDS-PAGE Clarkia breweri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.25 geranyl diphosphate + H2O Clarkia breweri
-
(3S)-linalool + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.25 Clarkia breweri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.25 native enzyme 26fold from stigmata of freshly opened flowers by anion exchange and hydroxyapatite chromatography to over 95% purity Clarkia breweri

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.25 geranyl diphosphate + H2O = (3S)-linalool + diphosphate reaction mechanism Clarkia breweri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.25 stigma
-
Clarkia breweri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.25 0.0004
-
purified enzyme Clarkia breweri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.25 (S)-linalyl diphosphate + H2O low activity, 15% of the maximal activity with geranyl diphosphate Clarkia breweri (3S)-linalool + diphosphate
-
?
4.2.3.25 geranyl diphosphate + H2O
-
Clarkia breweri (3S)-linalool + diphosphate
-
?
4.2.3.25 geranyl diphosphate + H2O enantiospecific reaction Clarkia breweri (3S)-linalool + diphosphate product identification ?
4.2.3.25 additional information no activity with (R)-linalyl diphosphate Clarkia breweri ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.3.25 monomer 1 * 79000, SDS-PAGE Clarkia breweri

Synonyms

EC Number Synonyms Comment Organism
4.2.3.25 LIS
-
Clarkia breweri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.25 20
-
assay at Clarkia breweri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.25 7.4
-
broad optimum Clarkia breweri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.25 6.4 8.4 80% of maximal activity within this range Clarkia breweri