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Literature summary extracted from

  • Nashed, N.T.; Michaud, D.P.; Levin, W.; Jerina, D.M.
    Properties of liver microsomal cholesterol 5,6-oxide hydrolase (1985), Arch. Biochem. Biophys., 241, 149-162.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.3.2.11 acetonitrile activates, maximal at 6.25% v/v Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.11 5,6alpha-imino-5alpha-cholestan-3beta-ol strong inhibition Rattus norvegicus
3.3.2.11 9,10-iminophenanthrene
-
Rattus norvegicus
3.3.2.11 cholestane-3beta,5alpha,6alpha-triol IC50 is 0.110 mM Rattus norvegicus
3.3.2.11 cholestane-3beta,5alpha,6beta-triol IC50 is 0.03 mM Rattus norvegicus
3.3.2.11 cholestane-3beta,5beta,6alpha-triol IC50 is 0.085 mM Rattus norvegicus
3.3.2.11 cholestane-3beta,5beta,6beta-triol IC50 is 0.118 mM Rattus norvegicus
3.3.2.11 cholesterol IC50 is above 0.125 mM Rattus norvegicus
3.3.2.11 Iminosqualene
-
Rattus norvegicus
3.3.2.11 oxidosqualene IC50 is above 0.16 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.11 additional information
-
additional information kinetics Mus musculus
3.3.2.11 additional information
-
additional information kinetics Homo sapiens
3.3.2.11 additional information
-
additional information kinetics Rattus norvegicus
3.3.2.11 additional information
-
additional information kinetics Oryctolagus cuniculus
3.3.2.11 additional information
-
additional information kinetics Mesocricetus auratus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.11 microsome
-
Mus musculus
-
-
3.3.2.11 microsome
-
Homo sapiens
-
-
3.3.2.11 microsome
-
Rattus norvegicus
-
-
3.3.2.11 microsome
-
Oryctolagus cuniculus
-
-
3.3.2.11 microsome
-
Mesocricetus auratus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O Mus musculus
-
cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O Homo sapiens
-
cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O Rattus norvegicus
-
cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O Oryctolagus cuniculus
-
cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O Mesocricetus auratus
-
cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O Rattus norvegicus
-
cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 additional information Mus musculus the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity ?
-
?
3.3.2.11 additional information Homo sapiens the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity ?
-
?
3.3.2.11 additional information Rattus norvegicus the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity ?
-
?
3.3.2.11 additional information Oryctolagus cuniculus the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity ?
-
?
3.3.2.11 additional information Mesocricetus auratus the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.11 Homo sapiens
-
-
-
3.3.2.11 Mesocricetus auratus
-
-
-
3.3.2.11 Mus musculus
-
-
-
3.3.2.11 Oryctolagus cuniculus
-
-
-
3.3.2.11 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.11 liver microsome preparation Mus musculus
3.3.2.11 liver microsome preparation Homo sapiens
3.3.2.11 liver microsome preparation Rattus norvegicus
3.3.2.11 liver microsome preparation Oryctolagus cuniculus
3.3.2.11 liver microsome preparation Mesocricetus auratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.11 liver
-
Mus musculus
-
3.3.2.11 liver
-
Homo sapiens
-
3.3.2.11 liver
-
Rattus norvegicus
-
3.3.2.11 liver
-
Oryctolagus cuniculus
-
3.3.2.11 liver
-
Mesocricetus auratus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3.2.11 0.00004
-
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol Oryctolagus cuniculus
3.3.2.11 0.0001
-
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol Mesocricetus auratus
3.3.2.11 0.00014
-
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Oryctolagus cuniculus
3.3.2.11 0.00018
-
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol Mus musculus
3.3.2.11 0.0002
-
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Mesocricetus auratus
3.3.2.11 0.00038
-
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol Homo sapiens
3.3.2.11 0.00051
-
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Mus musculus
3.3.2.11 0.00052
-
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus
3.3.2.11 0.00108
-
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Homo sapiens
3.3.2.11 0.00108
-
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
Mus musculus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
Homo sapiens cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
Oryctolagus cuniculus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
Mesocricetus auratus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Mus musculus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Homo sapiens cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Oryctolagus cuniculus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Mesocricetus auratus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O
-
Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Mus musculus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Homo sapiens cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Oryctolagus cuniculus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Mesocricetus auratus cholestane-3beta,5alpha,6beta-triol
-
?
3.3.2.11 additional information the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity Mus musculus ?
-
?
3.3.2.11 additional information the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity Homo sapiens ?
-
?
3.3.2.11 additional information the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity Rattus norvegicus ?
-
?
3.3.2.11 additional information the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity Oryctolagus cuniculus ?
-
?
3.3.2.11 additional information the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity Mesocricetus auratus ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.11 37
-
assay at Mus musculus
3.3.2.11 37
-
assay at Homo sapiens
3.3.2.11 37
-
assay at Rattus norvegicus
3.3.2.11 37
-
assay at Oryctolagus cuniculus
3.3.2.11 37
-
assay at Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.11 7.4
-
assay at Mus musculus
3.3.2.11 7.4
-
assay at Homo sapiens
3.3.2.11 7.4
-
assay at Rattus norvegicus
3.3.2.11 7.4
-
assay at Oryctolagus cuniculus
3.3.2.11 7.4
-
assay at Mesocricetus auratus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.3.2.11 0.000085
-
5,6alpha-imino-5alpha-cholestan-3beta-ol pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus
3.3.2.11 0.0366
-
Iminosqualene pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus
3.3.2.11 0.126
-
9,10-iminophenanthrene pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.3.2.11 0.03
-
IC50 is 0.03 mM Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
3.3.2.11 0.085
-
IC50 is 0.085 mM Rattus norvegicus cholestane-3beta,5beta,6alpha-triol
3.3.2.11 0.11
-
IC50 is 0.110 mM Rattus norvegicus cholestane-3beta,5alpha,6alpha-triol
3.3.2.11 0.118
-
IC50 is 0.118 mM Rattus norvegicus cholestane-3beta,5beta,6beta-triol
3.3.2.11 0.125
-
IC50 is above 0.125 mM Rattus norvegicus cholesterol
3.3.2.11 0.16
-
IC50 is above 0.16 mM Rattus norvegicus oxidosqualene