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Literature summary extracted from

  • Jorgensen, F.; Hansen, O.C.; Stougaard, P.
    Enzymatic conversion of D-galactose to D-tagatose: heterologous expression and characterisation of a thermostable L-arabinose isomerase from Thermoanaerobacter mathranii (2004), Appl. Microbiol. Biotechnol., 64, 816-822.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.4 nutrition the product D-tagatose is currently introduced as a low-calorie bulk sweetener Thermoanaerobacter mathranii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli Thermotoga maritima
5.3.1.4 expression in Escherichia coli Thermoanaerobacter mathranii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 80
-
 L-arabinose
-
 Thermoanaerobacter mathranii
5.3.1.4 120
-
 D-galactose
-
 Thermoanaerobacter mathranii
5.3.1.4 145
-
 D-fucose
-
 Thermoanaerobacter mathranii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ addition of CoSO4 reactivates the enzyme to 45% after inactivation with EDTA Thermoanaerobacter mathranii
5.3.1.4 Co2+ preferred cofactor Thermotoga maritima
5.3.1.4 Fe2+ addition of FeSO4 reactivates the enzyme to 76% after inactivation with EDTA Thermoanaerobacter mathranii
5.3.1.4 Mn2+ addition of MnCl2 reactivates the enzyme to 100% after inactivation with EDTA Thermoanaerobacter mathranii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 52000
-
 monomer, SDS-PAGE Thermoanaerobacter mathranii
5.3.1.4 52820
-
 monomer, calculated from amino acid sequence Thermoanaerobacter mathranii
5.3.1.4 55000
-
 monomer, SDS-PAGE Thermotoga maritima
5.3.1.4 212000
-
 gel filtration Thermotoga maritima
5.3.1.4 220000
-
 gel filtration Thermoanaerobacter mathranii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 D-galactose Thermotoga maritima
-
 D-tagatose
-
 ?
5.3.1.4 D-galactose Thermoanaerobacter mathranii
-
 D-tagatose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Thermoanaerobacter mathranii Q70G56 araA
-
5.3.1.4 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 recombinant enzyme purified from Escherichia coli Thermotoga maritima
5.3.1.4 recombinant enzyme purified from Escherichia coli Thermoanaerobacter mathranii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 0.055
-
 recovered activity after immobilization of the enzyme Thermoanaerobacter mathranii
5.3.1.4 4
-
 after purification to homogeneity Thermotoga maritima
5.3.1.4 4
-
 after purification to homogeneity Thermoanaerobacter mathranii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-Fucose
-
 Thermoanaerobacter mathranii ?
-
 ?
5.3.1.4 D-galactose
-
 Thermotoga maritima D-tagatose
-
 ?
5.3.1.4 D-galactose
-
 Thermoanaerobacter mathranii D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Thermoanaerobacter mathranii L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 tetramer 4 * 52000, SDS-PAGE, gel filtration Thermoanaerobacter mathranii
5.3.1.4 tetramer 4 * 55000, SDS-PAGE, gel filtration Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 L-arabinose isomerase
-
 Thermotoga maritima
5.3.1.4 L-arabinose isomerase
-
 Thermoanaerobacter mathranii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 65
-
-
 Thermoanaerobacter mathranii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 8
-
-
 Thermoanaerobacter mathranii