EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.4 | expression in Escherichia coli | Halalkalibacterium halodurans |
5.3.1.4 | expression in Escherichia coli | Alicyclobacillus acidocaldarius |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.1.4 | E268K | pH optimum shifts from pH 8 to 7 | Halalkalibacterium halodurans |
5.3.1.4 | K269E | pH optimum shifts from pH 6 to 7 | Alicyclobacillus acidocaldarius |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.4 | EDTA | 1 mM inhibits activity completely | Alicyclobacillus acidocaldarius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.4 | 38.2 | - |
L-arabinose | wild-type at pH 6 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 39.7 | - |
L-arabinose | wild-type at pH 7 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 43.7 | - |
L-arabinose | mutant E268K at pH 7 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 43.8 | - |
L-arabinose | wild-type at pH 8 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 46 | - |
L-arabinose | mutant E268K at pH 8 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 46.2 | - |
L-arabinose | wild-type at pH 7 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 47.5 | - |
L-arabinose | wild-type at pH 8 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 48 | - |
L-arabinose | wild-type at pH 6 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 52.5 | - |
L-arabinose | mutant K269E at pH 8 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 53.8 | - |
L-arabinose | mutant K269E at pH 7 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 56.1 | - |
L-arabinose | mutant E268K at pH 6 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 57.9 | - |
L-arabinose | mutant K269E at pH 6 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 129 | - |
D-galactose | wild-type at pH 6 and 65°C | Alicyclobacillus acidocaldarius |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.4 | Co2+ | 1 mM required for activity | Alicyclobacillus acidocaldarius | |
5.3.1.4 | Mg2+ | 1 mM required for activity | Alicyclobacillus acidocaldarius | |
5.3.1.4 | Mn2+ | 1 mM required for activity | Alicyclobacillus acidocaldarius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 56040 | - |
monomer, calculated from amino acid sequence | Alicyclobacillus acidocaldarius |
5.3.1.4 | 57000 | - |
monomer, SDS-PAGE | Alicyclobacillus acidocaldarius |
5.3.1.4 | 224000 | - |
gel filtration | Alicyclobacillus acidocaldarius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.4 | D-galactose | Halalkalibacterium halodurans | - |
D-tagatose | - |
? | |
5.3.1.4 | D-galactose | Alicyclobacillus acidocaldarius | - |
D-tagatose | - |
? | |
5.3.1.4 | L-arabinose | Halalkalibacterium halodurans | - |
L-ribulose | - |
? | |
5.3.1.4 | L-arabinose | Alicyclobacillus acidocaldarius | - |
L-ribulose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.4 | Alicyclobacillus acidocaldarius | Q2VMT2 | - |
- |
5.3.1.4 | Halalkalibacterium halodurans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.4 | - |
Halalkalibacterium halodurans |
5.3.1.4 | recombinant enzyme is purified to homogeneity by heat treatment, ion-exchange chromatography, and gel filtration | Alicyclobacillus acidocaldarius |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 7.5 | - |
wild-type with D-galactose as substrate at pH 6 and 65°C | Alicyclobacillus acidocaldarius |
5.3.1.4 | 17 | - |
wild-type with L-arabinose as substrate at pH 6 and 50°C | Halalkalibacterium halodurans |
5.3.1.4 | 21 | - |
mutant K269E with L-arabinose as substrate at pH 6 and 65°C | Alicyclobacillus acidocaldarius |
5.3.1.4 | 25.3 | - |
wild-type with L-arabinose as substrate at pH 7 and 50°C | Halalkalibacterium halodurans |
5.3.1.4 | 25.8 | - |
mutant E268K with L-arabinose as substrate at pH 6 and 50°C | Halalkalibacterium halodurans |
5.3.1.4 | 26.8 | - |
wild-type with L-arabinose as substrate at pH 8 and 65°C | Alicyclobacillus acidocaldarius |
5.3.1.4 | 27.6 | - |
mutant K269E with L-arabinose as substrate at pH 8 and 65°C | Alicyclobacillus acidocaldarius |
5.3.1.4 | 30.6 | - |
wild-type with L-arabinose as substrate at pH 7 and 65°C | Alicyclobacillus acidocaldarius |
5.3.1.4 | 31.7 | - |
mutant E268K with L-arabinose as substrate at pH 8 and 50°C | Halalkalibacterium halodurans |
5.3.1.4 | 33.2 | - |
wild-type with L-arabinose as substrate at pH 8 and 50°C | Halalkalibacterium halodurans |
5.3.1.4 | 35.3 | - |
mutant E268K with L-arabinose as substrate at pH 7 and 50°C | Halalkalibacterium halodurans |
5.3.1.4 | 35.5 | - |
wild-type with L-arabinose as substrate at pH 6 and 65°C | Alicyclobacillus acidocaldarius |
5.3.1.4 | 39.9 | - |
mutant K269E with L-arabinose as substrate at pH 7 and 65°C | Alicyclobacillus acidocaldarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.4 | D-galactose | - |
Halalkalibacterium halodurans | D-tagatose | - |
? | |
5.3.1.4 | D-galactose | - |
Alicyclobacillus acidocaldarius | D-tagatose | - |
? | |
5.3.1.4 | L-arabinose | - |
Halalkalibacterium halodurans | L-ribulose | - |
? | |
5.3.1.4 | L-arabinose | - |
Alicyclobacillus acidocaldarius | L-ribulose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.4 | tetramer | alpha4, 4 * 57000, SDS-PAGE, gel filtration | Alicyclobacillus acidocaldarius |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.4 | AI | - |
Halalkalibacterium halodurans |
5.3.1.4 | AI | - |
Alicyclobacillus acidocaldarius |
5.3.1.4 | L-arabinose isomerase | - |
Halalkalibacterium halodurans |
5.3.1.4 | L-arabinose isomerase | - |
Alicyclobacillus acidocaldarius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 50 | - |
- |
Halalkalibacterium halodurans |
5.3.1.4 | 65 | - |
- |
Alicyclobacillus acidocaldarius |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.4 | 15.88 | - |
L-arabinose | wild-type at pH 6 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 19.62 | - |
L-arabinose | mutant K269E at pH 6 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 23.65 | - |
L-arabinose | wild-type at pH 7 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 24.11 | - |
L-arabinose | mutant E268K at pH 6 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 24.97 | - |
L-arabinose | wild-type at pH 8 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 25.8 | - |
L-arabinose | mutant K269E at pH 8 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 28.55 | - |
L-arabinose | wild-type at pH 7 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 29.63 | - |
L-arabinose | mutant E268K at pH 8 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 31.03 | - |
L-arabinose | wild-type at pH 8 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 32.93 | - |
L-arabinose | mutant E268K at pH 7 and 50°C | Halalkalibacterium halodurans | |
5.3.1.4 | 33.15 | - |
L-arabinose | wild-type at pH 6 and 65°C | Alicyclobacillus acidocaldarius | |
5.3.1.4 | 37.2 | - |
L-arabinose | mutant K269E at pH 7 and 65°C | Alicyclobacillus acidocaldarius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 6 | 6.5 | wild-type | Alicyclobacillus acidocaldarius |
5.3.1.4 | 7 | - |
mutant E268K | Halalkalibacterium halodurans |
5.3.1.4 | 7 | - |
mutant K269E | Alicyclobacillus acidocaldarius |
5.3.1.4 | 7.5 | 8 | wild-type | Halalkalibacterium halodurans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 5.2 | 8 | more than 50% of the maximal activity | Alicyclobacillus acidocaldarius |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 5 | - |
half-life: about 10 h | Alicyclobacillus acidocaldarius |
5.3.1.4 | 6 | - |
AI retains its original activity even after 48 h incubation | Alicyclobacillus acidocaldarius |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
5.3.1.4 | Alicyclobacillus acidocaldarius | isoelectric focusing | - |
5 |
5.3.1.4 | Alicyclobacillus acidocaldarius | calculated | - |
5.51 |