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Literature summary extracted from

  • Lee, S.J.; Lee, D.W.; Choe, E.A.; Hong, Y.H.; Kim, S.B.; Kim, B.C.; Pyun, Y.R.
    Characterization of a thermoacidophilic L-arabinose isomerase from Alicyclobacillus acidocaldarius: role of Lys-269 in pH optimum (2005), Appl. Environ. Microbiol., 71, 7888-7896.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli Halalkalibacterium halodurans
5.3.1.4 expression in Escherichia coli Alicyclobacillus acidocaldarius

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.4 E268K pH optimum shifts from pH 8 to 7 Halalkalibacterium halodurans
5.3.1.4 K269E pH optimum shifts from pH 6 to 7 Alicyclobacillus acidocaldarius

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 EDTA 1 mM inhibits activity completely Alicyclobacillus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 38.2
-
L-arabinose wild-type at pH 6 and 50°C Halalkalibacterium halodurans
5.3.1.4 39.7
-
L-arabinose wild-type at pH 7 and 50°C Halalkalibacterium halodurans
5.3.1.4 43.7
-
L-arabinose mutant E268K at pH 7 and 50°C Halalkalibacterium halodurans
5.3.1.4 43.8
-
L-arabinose wild-type at pH 8 and 50°C Halalkalibacterium halodurans
5.3.1.4 46
-
L-arabinose mutant E268K at pH 8 and 50°C Halalkalibacterium halodurans
5.3.1.4 46.2
-
L-arabinose wild-type at pH 7 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 47.5
-
L-arabinose wild-type at pH 8 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 48
-
L-arabinose wild-type at pH 6 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 52.5
-
L-arabinose mutant K269E at pH 8 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 53.8
-
L-arabinose mutant K269E at pH 7 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 56.1
-
L-arabinose mutant E268K at pH 6 and 50°C Halalkalibacterium halodurans
5.3.1.4 57.9
-
L-arabinose mutant K269E at pH 6 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 129
-
D-galactose wild-type at pH 6 and 65°C Alicyclobacillus acidocaldarius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ 1 mM required for activity Alicyclobacillus acidocaldarius
5.3.1.4 Mg2+ 1 mM required for activity Alicyclobacillus acidocaldarius
5.3.1.4 Mn2+ 1 mM required for activity Alicyclobacillus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 56040
-
monomer, calculated from amino acid sequence Alicyclobacillus acidocaldarius
5.3.1.4 57000
-
monomer, SDS-PAGE Alicyclobacillus acidocaldarius
5.3.1.4 224000
-
gel filtration Alicyclobacillus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 D-galactose Halalkalibacterium halodurans
-
D-tagatose
-
?
5.3.1.4 D-galactose Alicyclobacillus acidocaldarius
-
D-tagatose
-
?
5.3.1.4 L-arabinose Halalkalibacterium halodurans
-
L-ribulose
-
?
5.3.1.4 L-arabinose Alicyclobacillus acidocaldarius
-
L-ribulose
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Alicyclobacillus acidocaldarius Q2VMT2
-
-
5.3.1.4 Halalkalibacterium halodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4
-
Halalkalibacterium halodurans
5.3.1.4 recombinant enzyme is purified to homogeneity by heat treatment, ion-exchange chromatography, and gel filtration Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 7.5
-
wild-type with D-galactose as substrate at pH 6 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 17
-
wild-type with L-arabinose as substrate at pH 6 and 50°C Halalkalibacterium halodurans
5.3.1.4 21
-
mutant K269E with L-arabinose as substrate at pH 6 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 25.3
-
wild-type with L-arabinose as substrate at pH 7 and 50°C Halalkalibacterium halodurans
5.3.1.4 25.8
-
mutant E268K with L-arabinose as substrate at pH 6 and 50°C Halalkalibacterium halodurans
5.3.1.4 26.8
-
wild-type with L-arabinose as substrate at pH 8 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 27.6
-
mutant K269E with L-arabinose as substrate at pH 8 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 30.6
-
wild-type with L-arabinose as substrate at pH 7 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 31.7
-
mutant E268K with L-arabinose as substrate at pH 8 and 50°C Halalkalibacterium halodurans
5.3.1.4 33.2
-
wild-type with L-arabinose as substrate at pH 8 and 50°C Halalkalibacterium halodurans
5.3.1.4 35.3
-
mutant E268K with L-arabinose as substrate at pH 7 and 50°C Halalkalibacterium halodurans
5.3.1.4 35.5
-
wild-type with L-arabinose as substrate at pH 6 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 39.9
-
mutant K269E with L-arabinose as substrate at pH 7 and 65°C Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
Halalkalibacterium halodurans D-tagatose
-
?
5.3.1.4 D-galactose
-
Alicyclobacillus acidocaldarius D-tagatose
-
?
5.3.1.4 L-arabinose
-
Halalkalibacterium halodurans L-ribulose
-
?
5.3.1.4 L-arabinose
-
Alicyclobacillus acidocaldarius L-ribulose
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 tetramer alpha4, 4 * 57000, SDS-PAGE, gel filtration Alicyclobacillus acidocaldarius

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 AI
-
Halalkalibacterium halodurans
5.3.1.4 AI
-
Alicyclobacillus acidocaldarius
5.3.1.4 L-arabinose isomerase
-
Halalkalibacterium halodurans
5.3.1.4 L-arabinose isomerase
-
Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 50
-
-
Halalkalibacterium halodurans
5.3.1.4 65
-
-
Alicyclobacillus acidocaldarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.4 15.88
-
L-arabinose wild-type at pH 6 and 50°C Halalkalibacterium halodurans
5.3.1.4 19.62
-
L-arabinose mutant K269E at pH 6 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 23.65
-
L-arabinose wild-type at pH 7 and 50°C Halalkalibacterium halodurans
5.3.1.4 24.11
-
L-arabinose mutant E268K at pH 6 and 50°C Halalkalibacterium halodurans
5.3.1.4 24.97
-
L-arabinose wild-type at pH 8 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 25.8
-
L-arabinose mutant K269E at pH 8 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 28.55
-
L-arabinose wild-type at pH 7 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 29.63
-
L-arabinose mutant E268K at pH 8 and 50°C Halalkalibacterium halodurans
5.3.1.4 31.03
-
L-arabinose wild-type at pH 8 and 50°C Halalkalibacterium halodurans
5.3.1.4 32.93
-
L-arabinose mutant E268K at pH 7 and 50°C Halalkalibacterium halodurans
5.3.1.4 33.15
-
L-arabinose wild-type at pH 6 and 65°C Alicyclobacillus acidocaldarius
5.3.1.4 37.2
-
L-arabinose mutant K269E at pH 7 and 65°C Alicyclobacillus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 6 6.5 wild-type Alicyclobacillus acidocaldarius
5.3.1.4 7
-
mutant E268K Halalkalibacterium halodurans
5.3.1.4 7
-
mutant K269E Alicyclobacillus acidocaldarius
5.3.1.4 7.5 8 wild-type Halalkalibacterium halodurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.4 5.2 8 more than 50% of the maximal activity Alicyclobacillus acidocaldarius

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.4 5
-
half-life: about 10 h Alicyclobacillus acidocaldarius
5.3.1.4 6
-
AI retains its original activity even after 48 h incubation Alicyclobacillus acidocaldarius

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.4 Alicyclobacillus acidocaldarius isoelectric focusing
-
5
5.3.1.4 Alicyclobacillus acidocaldarius calculated
-
5.51