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Literature summary extracted from

  • Frobert, E.; Ooka, T.; Cortay, J.C.; Lina, B.; Thouvenot, D.; Morfin, F.
    Herpes simplex virus thymidine kinase mutations associated with resistance to acyclovir: a site-directed mutagenesis study (2005), Antimicrob. Agents Chemother., 49, 1055-1059.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Human alphaherpesvirus 1

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.21 A175V site-sirected mutagenesis, inactive mutant, mutation of residue 51 might in vivo be involved into generation of acyclovir resistance of HSV Human alphaherpesvirus 1
2.7.1.21 D77N site-sirected mutagenesis, mutant activity is slightly increased compared to the wild-type enzyme Human alphaherpesvirus 1
2.7.1.21 E83K site-sirected mutagenesis, inactive mutant, mutation of residue 51 might in vivo be involved into generation of acyclovir resistance of HSV Human alphaherpesvirus 1
2.7.1.21 R51W site-sirected mutagenesis, inactive mutant, mutation of residue 51 might in vivo be involved into generation of acyclovir resistance of HSV Human alphaherpesvirus 1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Mg2+
-
 Human alphaherpesvirus 1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.21 ATP + acyclovir Human alphaherpesvirus 1 drug activation by phosphorylation, some naturally occurring mutations lead to inability of acyclovir phosphorylation and thus resistance against the drug ADP + acyclovir phosphate
-
 ?
2.7.1.21 ATP + thymidine Human alphaherpesvirus 1
-
 ADP + thymidine 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Human alphaherpesvirus 1
-
 i.e. HSV-1, acyclovir-sensitive strain KOS, genetic polymorphism
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Human alphaherpesvirus 1

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.21 ATP + thymidine = ADP + dTMP the amino acid residues at positions 51, 83, and 185 are important for enzyme activity Human alphaherpesvirus 1
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + acyclovir drug activation by phosphorylation Human alphaherpesvirus 1 ADP + acyclovir phosphate
-
 ?
2.7.1.21 ATP + acyclovir drug activation by phosphorylation, some naturally occurring mutations lead to inability of acyclovir phosphorylation and thus resistance against the drug Human alphaherpesvirus 1 ADP + acyclovir phosphate
-
 ?
2.7.1.21 ATP + thymidine
-
 Human alphaherpesvirus 1 ADP + thymidine 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 TK
-
 Human alphaherpesvirus 1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.21 37
-
 assay at Human alphaherpesvirus 1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 7.5
-
 assay at Human alphaherpesvirus 1

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.21 ATP
-
 Human alphaherpesvirus 1