EC Number | Cloned (Comment) | Organism |
---|---|---|
5.6.2.2 | DNA sequence determination, mapping and analysis of drug-induced DNA cleavage, expression of wild-type and mutant enzymes in strain JM109 | Escherichia coli K-12 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.6.2.2 | S83L | site-directed mutagenesis, A subunit residue, the mutation leads to enzyme resistance against fluoroquinolones, but increased sensitivity to epipodophyllotoxins and amino-azatoxin in vivo in strain RFM443-242 | Escherichia coli K-12 |
5.6.2.2 | S83W | site-directed mutagenesis, A subunit residue, the mutation leads to enzyme resistance against fluoroquinolones, but increased sensitivity to epipodophyllotoxins and amino-azatoxin in vivo in strain RFM443-242 | Escherichia coli K-12 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.2 | 11beta-(2''-N,N-dimethylaminoethyl)-amino-azatoxin | belongs to the epipodophyllotoxins, inhibitors of eukaryotic enzymes, inhibits the S83W mutant enzyme, but not the wild-type bacterial enzyme | Escherichia coli K-12 | |
5.6.2.2 | Ciprofloxacin | inhibits wild-type enzyme completely at 0.01 mM, 50% inhibition of the mutant S83W at about 0.065 mM | Escherichia coli K-12 | |
5.6.2.2 | etoposide | belongs to the epipodophyllotoxins, inhibitors of eukaryotic enzymes, inhibits the S83W mutant enzyme, but not the wild-type bacterial enzyme | Escherichia coli K-12 | |
5.6.2.2 | fluoroquinolones | broad-spectrum antimicrobial agents, Ser83 is responsible for sensitivity, mutation S83W and S83L lead to resistance against the agents | Escherichia coli K-12 | |
5.6.2.2 | Teniposide | belongs to the epipodophyllotoxins, inhibitors of eukaryotic enzymes, inhibits the S83W mutant enzyme, but not the wild-type bacterial enzyme | Escherichia coli K-12 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.2.2 | Escherichia coli K-12 | - |
gene gyrA encoding the A subunit | - |
5.6.2.2 | Escherichia coli K-12 RFM443 | - |
gene gyrA encoding the A subunit | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.6.2.2 | additional information | - |
- |
Escherichia coli K-12 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.2.2 | DNA gyrase | - |
Escherichia coli K-12 |
5.6.2.2 | GyrA | - |
Escherichia coli K-12 |
5.6.2.2 | type II topoisomerase | - |
Escherichia coli K-12 |