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Literature summary extracted from

  • Chen, W.T.; Liu, M.C.; Yang, Y.S.
    Fluorometric assay for alcohol sulfotransferase (2005), Anal. Biochem., 339, 54-60.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Escherichia coli
-
-
-
2.8.2.2 Rattus norvegicus
-
-
-
2.8.2.2 Sus scrofa
-
-
-
2.8.2.14 Homo sapiens
-
-
-
3.1.6.1 Escherichia coli
-
 BL-21 strain
-
3.1.6.1 Escherichia coli BL-21
-
 BL-21 strain
-
3.1.6.1 Rattus norvegicus
-
-
-
3.1.6.1 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 liver
-
 Rattus norvegicus
-
2.8.2.2 liver
-
 Sus scrofa
-
2.8.2.14 liver
-
 Homo sapiens
-
3.1.6.1 liver
-
 Rattus norvegicus
-
3.1.6.1 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.2 additional information
-
 continuous fluorimetric assay for alcohol sulfotransferase whose sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader Escherichia coli
2.8.2.2 additional information
-
 continuous fluorimetric assay for alcohol sulfotransferase whose sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader Sus scrofa
2.8.2.2 additional information
-
 continuous fluorimetric assay for alcohol sulfotransferase whoses sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader Rattus norvegicus
2.8.2.14 additional information
-
 continuous fluorimetric assay for alcohol sulfotransferase whoses sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader Homo sapiens
3.1.6.1 additional information
-
 0.0006 micromol/min/g wet cells of E. coli, at pH 7.0 with 4 mM methylumblliferyl sulfate Escherichia coli
3.1.6.1 additional information
-
 0.0008 micromol/min/g wet cells of E. coli overexpressing a human alcohol sulfotransferase, at pH 7.0 with 4 mM methylumblliferyl sulfate Escherichia coli
3.1.6.1 additional information
-
 0.0008 micromol/min/g wet cells of liver, at pH 7.0 with 4 mM methylumblliferyl sulfate Sus scrofa
3.1.6.1 additional information
-
 0.0056 micromol/min/g wet cells of liver, at pH 7.0 with 4 mM methylumblliferyl sulfate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Sus scrofa adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Escherichia coli adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Escherichia coli BL-21 adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3.1.6.1 4-methylumbelliferyl sulfate + H2O
-
 Escherichia coli 4-methylumbelliferol + sulfate
-
 ?
3.1.6.1 4-methylumbelliferyl sulfate + H2O
-
 Rattus norvegicus 4-methylumbelliferol + sulfate
-
 ?
3.1.6.1 4-methylumbelliferyl sulfate + H2O
-
 Sus scrofa 4-methylumbelliferol + sulfate
-
 ?
3.1.6.1 4-methylumbelliferyl sulfate + H2O
-
 Escherichia coli BL-21 4-methylumbelliferol + sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.2 alcohol sulfotransferase
-
 Escherichia coli
2.8.2.2 alcohol sulfotransferase
-
 Rattus norvegicus
2.8.2.2 alcohol sulfotransferase
-
 Sus scrofa
2.8.2.2 AST
-
 Escherichia coli
2.8.2.2 AST
-
 Rattus norvegicus
2.8.2.2 AST
-
 Sus scrofa
2.8.2.14 dehydroepiandrosterone sulfotransferase
-
 Homo sapiens
3.1.6.1 arylsulfatase
-
 Escherichia coli
3.1.6.1 arylsulfatase
-
 Rattus norvegicus
3.1.6.1 arylsulfatase
-
 Sus scrofa