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Literature summary extracted from

  • Fujita, A.; Sato, C.; Munster-Kuhnel, A.K.; Gerardy-Schahn, R.; Kitajima, K.
    Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system (2005), Anal. Biochem., 337, 12-21.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.43 K198A mutant enzyme is active at moderately reduced level Mus musculus
2.7.7.43 R199A inactive mutant protein Mus musculus
2.7.7.43 R199A/R202A inactive mutant protein Mus musculus
2.7.7.43 R201A mutant enzyme is active at moderately reduced level Mus musculus
2.7.7.43 R202A mutant enzyme shows drastically reduced activity Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Mus musculus
-
 recombinant
-
2.7.7.43 Oncorhynchus mykiss
-
 recombinant
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.43 additional information
-
 simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system Mus musculus
2.7.7.43 additional information
-
 simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + deaminoneuraminic acid
-
 Oncorhynchus mykiss ?
-
 ?
2.7.7.43 CTP + N-acylneuraminate enzyme is equally active with either of the three substrates: N-acylneuraminate, N-glycolylneuraminate and deaminoneuraminic acid Oncorhynchus mykiss diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate enzyme shows pronounced specificity for N-acylneuraminate Mus musculus diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-glycolylneuraminate
-
 Oncorhynchus mykiss diphosphate + CMP-N-glycolylneuraminate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.43 CMP-Sia synthetase
-
 Mus musculus
2.7.7.43 CMP-Sia synthetase
-
 Oncorhynchus mykiss
2.7.7.43 CSS
-
 Mus musculus
2.7.7.43 CSS
-
 Oncorhynchus mykiss
2.7.7.43 cytidine monophosphate sialic acid synthetase
-
 Mus musculus
2.7.7.43 cytidine monophosphate sialic acid synthetase
-
 Oncorhynchus mykiss