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Literature summary extracted from

  • Tamada, T.; Feese, M.D.; Ferri, S.R.; Kato, Y.; Yajima, R.; Toguri, T.; Kuroki, R.
    Substrate recognition and selectivity of plant glycerol-3-phosphate acyltransferases (GPATs) from Cucurbita moschata and Spinacia oleracea (2004), Acta Crystallogr. Sect. D, 60, 13-21.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 expression of chimeric mature enzyme containing residues 128-187 from Spinacia oleracea and residues 1-127 from Cucurbita moscata, in Escherichia coli strain BL21(DE3) Spinacia oleracea
2.3.1.15 expression of native and selenomethionine-labeled wild-type and chimeric mature enzymes, the latter contains residues 128-187 from Spinacia oleracea and residues 1-127 from Cucurbita moscata, in Escherichia coli strain BL21(DE3) and a nonauxotrophic strain Cucurbita moschata

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.15 purified recombinant native and selenomethionine-labeled wild-type and chimeric enzymes, 14 mg/ml protein in 20 mM sodium phosphate, pH 8.0, 4°C, hanging drop vapour diffusion method, crystallization solution contains 4-25% PEG 4000, 0.1 M ammonium acetate,10% v/v 2-propanolin 100 mM citrate, pH 5.6, X-ray diffraction structure determination and analysis at 1.55 A resolution Cucurbita moschata
2.3.1.15 recombinant chimeric enzyme, 14 mg/ml protein in 20 mM sodium phosphate, pH 8.0, 4°C, hanging drop vapour diffusion method, crystallization solution contains 4-25% PEG 4000, 0.1 M ammonium acetate,10% v/v 2-propanolin 100 mM citrate, pH 5.6, X-ray diffraction structure determination and analysis at 1.55 A resolution Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 chloroplast stroma Cucurbita moschata 9507
-
2.3.1.15 chloroplast stroma Spinacia oleracea 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Cucurbita moschata first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Spinacia oleracea first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 additional information Cucurbita moschata the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, squash is chilling-sensitive, overview ?
-
 ?
2.3.1.15 additional information Spinacia oleracea the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, spinach is chilling-insensitive, while squash is chilling-sensitive, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Cucurbita moschata
-
-
-
2.3.1.15 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 recombinant chimeric mature enzyme Spinacia oleracea
2.3.1.15 recombinant native and selenomethionine-labeled wild-type and chimeric mature enzymes by anio exchange chromatography Cucurbita moschata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes Cucurbita moschata CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Cucurbita moschata CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 additional information the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, squash is chilling-sensitive, overview Cucurbita moschata ?
-
 ?
2.3.1.15 additional information the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, spinach is chilling-insensitive, while squash is chilling-sensitive, overview Spinacia oleracea ?
-
 ?
2.3.1.15 additional information substrate specificity of wild-type and chimeric mutant enzymes, overview Cucurbita moschata ?
-
 ?
2.3.1.15 additional information substrate specificity of the chimeric mutant enzymes, overview Spinacia oleracea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase
-
 Cucurbita moschata
2.3.1.15 glycerol-3-phosphate acyltransferase
-
 Spinacia oleracea
2.3.1.15 GPAT
-
 Cucurbita moschata
2.3.1.15 GPAT
-
 Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 25
-
 assay at Cucurbita moschata
2.3.1.15 25
-
 assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7.4
-
 assay at Cucurbita moschata
2.3.1.15 7.4
-
 assay at Spinacia oleracea