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Literature summary extracted from

  • Takeishi, S.; Nakagawa, N.; Maoka, N.; Kihara, M.; Moriguchi, M.; Masui, R.; Kuramitsu, S.
    Crystallization and preliminary X-ray diffraction studies of nucleoside diphosphate kinase from Thermus thermophilus HB8 (2003), Acta Crystallogr. Sect. D, 59, 1843-1845.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expressed in Escherichia coli BL21(DE3)pLysE Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.6 hanging drop vapor diffusion method Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.6 0.02
-
 GDP pH 7.5, 25°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 15000
-
 4 * 15000, calculated from the deduced amino acid sequence, SDS-PAGE, native mass by gel filtration Thermus thermophilus
2.7.4.6 60000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + NDP Thermus thermophilus contributes to the maintenance of the cellular pools of all nucleosides triphosphates ADP + NTP
-
 ?
2.7.4.6 ATP + NDP Thermus thermophilus HB8 / ATCC 27634 / DSM 579 contributes to the maintenance of the cellular pools of all nucleosides triphosphates ADP + NTP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Thermus thermophilus
-
-
-
2.7.4.6 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 recombinant protein Thermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.6 ATP + nucleoside diphosphate = ADP + nucleoside triphosphate two-step ping-pong mechanism Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + CDP
-
 Thermus thermophilus ADP + CTP
-
 ?
2.7.4.6 ATP + CDP
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + CTP
-
 ?
2.7.4.6 ATP + GDP
-
 Thermus thermophilus ADP + GTP
-
 ?
2.7.4.6 ATP + GDP
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + GTP
-
 ?
2.7.4.6 ATP + NDP
-
 Thermus thermophilus ADP + NTP
-
 ?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates Thermus thermophilus ADP + NTP
-
 ?
2.7.4.6 ATP + NDP
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + NTP
-
 ?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + NTP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 tetramer 4 * 15000, calculated from the deduced amino acid sequence, SDS-PAGE, native mass by gel filtration Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.6 38
-
 GDP pH 7.5, 25 °C Thermus thermophilus