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Literature summary extracted from

  • Martin, P.; DeMel, S.; Shi, J.; Gladysheva, T.; Gatti, D.L.; Rosen, B.P.; Edwards, B.F.P.
    Insights into the structure, solvation, and mechanism of ArsC arsenate reductase, a novel arsenic detoxification enzyme (2001), Structure, 9, 1071-1081.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.20.99.1 free enzyme or enzyme complexed with arsenate and arsenite, X-ray diffraction structure determination and analysis at 1.65 A and 1.26 A, respecively Escherichia coli
1.20.99.1 in complex with arsenitate and arsenite and enzyme alone. Native structure shows sulfate and sulfite ions binding in the active site as analogs of arsenate and arsenite. Arsenate forms a covlaent adduct with C12 in the active site, showing tetrahedral geometry. The corresponding adduct with arsenite binds as thiarsahydroxy adduct. High number of 385 water molecules bound to crystal structure Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.20.99.1 sulfate binds at the active site as arsenate analogue Escherichia coli
1.20.99.1 sulfite binds at the active site as arsenite analogue Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.20.99.1 arsenate + reduced acceptor Escherichia coli detoxification enzyme, arsenite is extruded arsenite + acceptor
-
 ?
1.20.99.1 additional information Escherichia coli the arsRDABC plasmid operon codes for an ATP-dependent anion pump and is responsible for resistance against arsenate, arsenite, antimonite, and tellurite ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.20.99.1 Escherichia coli
-
 gene arsC, encoded on plasmid R773
-
1.20.99.1 Escherichia coli
-
 bearing plasmid R773 conferring resistance to arsenite, arsenate, antimonite, and tellurite
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.20.99.1 arsenite + acceptor + H2O = arsenate + reduced acceptor reaction mechanism, the catalytic Cys12, located at the active site, forms a covalent adduct with arsenate, the complex shows tetrahedral geometry, arsenite binds to Cys12 in a thiarsahydroxy adduct Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.99.1 arsenate + reduced acceptor
-
 Escherichia coli arsenite + acceptor
-
 ?
1.20.99.1 arsenate + reduced acceptor detoxification enzyme, arsenite is extruded Escherichia coli arsenite + acceptor
-
 ?
1.20.99.1 additional information the arsRDABC plasmid operon codes for an ATP-dependent anion pump and is responsible for resistance against arsenate, arsenite, antimonite, and tellurite Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.20.99.1 ArsC
-
 Escherichia coli
1.20.99.1 arsenate reductase
-
 Escherichia coli