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Literature summary extracted from

  • Solaas, K.; Sletta, R.J.; Soreide, O.; Kase, B.F.
    Presence of choloyl- and chenodeoxycholoyl-coenzyme A thioesterase activity in human liver (2000), Scand. J. Clin. Lab. Invest., 60, 91-102.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.27 CoA slight activation of activity with chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 DTT highly stimulating on the activity with both choloyl-CoA and chenodeoxycholoyl-CoA Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.27 CoA slight inhibition of activity with choloyl-CoA Homo sapiens
3.1.2.27 DTNB 75% inhibition at 0.3 mM, complete inhibition at 1.3 mM Homo sapiens
3.1.2.27 additional information substrate competition between choloyl-CoA and chenodeoxycholoyl-CoA, overview Homo sapiens
3.1.2.27 Phospholipids at high concentrations Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.27 additional information
-
 additional information
-
 Homo sapiens
3.1.2.27 0.011
-
 dithiothreitol pH 8.0, 37°C, with chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 0.018
-
 dithiothreitol pH 8.0, 37°C, with choloyl-CoA Homo sapiens
3.1.2.27 0.071
-
 chenodeoxycholoyl-CoA pH 8.0, 37°C, postnuclear fraction of liver homogenate Homo sapiens
3.1.2.27 0.077
-
 choloyl-CoA pH 8.0, 37°C, postnuclear fraction of liver homogenate Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.27 cytosol
-
 Homo sapiens 5829
-
3.1.2.27 microsome
-
 Homo sapiens
-
-
3.1.2.27 mitochondrion
-
 Homo sapiens 5739
-
3.1.2.27 additional information multiorganelle distribution, overview Homo sapiens
-
-
3.1.2.27 nucleus
-
 Homo sapiens 5634
-
3.1.2.27 peroxisome
-
 Homo sapiens 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.27 chenodeoxycholoyl-CoA + H2O Homo sapiens
-
 chenodeoxycholate + CoA free chenodeoxycholate activates the farnesoid X receptor ?
3.1.2.27 choloyl-CoA + H2O Homo sapiens
-
 cholate + CoA
-
 ?
3.1.2.27 additional information Homo sapiens the enzyme has a regulatory function in bile acid metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.27 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.27 partially, subcellular fractionation Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.27 choloyl-CoA + H2O = cholate + CoA the catalytic triad is formed by Asp233, Ser255, and Gln305 Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.27 liver
-
 Homo sapiens
-
3.1.2.27 liver healthy and metastatic Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.27 additional information
-
-
 Homo sapiens
3.1.2.27 0.00118
-
 nuclear fraction of liver homogenate, substrate choloyl-CoA Homo sapiens
3.1.2.27 0.00127
-
 cytosolic fraction of liver homogenate, substrate choloyl-CoA Homo sapiens
3.1.2.27 0.00186
-
 nuclear fraction of liver homogenate, substrate chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 0.00203
-
 microsomal fraction of liver homogenate, substrate chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 0.00203
-
 mitochondrial fraction of liver homogenate, substrate choloyl-CoA Homo sapiens
3.1.2.27 0.00227
-
 cytosolic fraction of liver homogenate, substrate chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 0.00247
-
 light mitochondrial fraction of liver homogenate, substrate choloyl-CoA Homo sapiens
3.1.2.27 0.00248
-
 microsomal fraction of liver homogenate, substrate choloyl-CoA Homo sapiens
3.1.2.27 0.00263
-
 mitochondrial fraction of liver homogenate, substrate chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 0.00276
-
 postnuclear fraction of liver homogenate, substrate choloyl-CoA Homo sapiens
3.1.2.27 0.00297
-
 postnuclear fraction of liver homogenate, substrate chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 0.00334
-
 light mitochondrial fraction of liver homogenate, substrate chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 0.0133
-
 peroxisomal fraction of a metastatic liver homogenate, substrate chenodeoxycholoyl-CoA Homo sapiens
3.1.2.27 0.01602
-
 peroxisomal fraction of a metastatic liver homogenate, substrate choloyl-CoA Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.27 chenodeoxycholoyl-CoA + H2O
-
 Homo sapiens chenodeoxycholate + CoA
-
 ?
3.1.2.27 chenodeoxycholoyl-CoA + H2O
-
 Homo sapiens chenodeoxycholate + CoA free chenodeoxycholate activates the farnesoid X receptor ?
3.1.2.27 chenodeoxycholoyl-CoA + H2O preferred substrate Homo sapiens chenodeoxycholate + CoA
-
 ?
3.1.2.27 choloyl-CoA + H2O
-
 Homo sapiens cholate + CoA
-
 ?
3.1.2.27 additional information the enzyme has a regulatory function in bile acid metabolism Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.27 chenodeoxycholic acid-CoA thioesterase
-
 Homo sapiens
3.1.2.27 chenodeoxycholoyl-coenzyme A thioesterase
-
 Homo sapiens
3.1.2.27 choloyl-coenzyme A thioesterase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.27 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.27 7 9 broad optimum with substrate choloyl-CoA Homo sapiens
3.1.2.27 7.5 9 broad optimum with substrate chenodeoxycholoyl-CoA Homo sapiens