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Literature summary extracted from

  • Qiu, X.; Choudhry, A.E.; Janson, C.A.; Grooms, M.; Daines, R.A.; Lonsdale, J.T.; Khandekar, S.S.
    Crystal structure and substrate specificity of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus (2005), Protein Sci., 14, 2087-2094.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 expression of N-terminally His2-tagged enzyme in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.41
-
 Staphylococcus aureus
2.3.1.180 15 mg/ml purified enzyme in 20 mM Tris, pH 8.0, 3 mM DTT, 100 mM NaCl, 10% glycerol, X-ray diffraction structure determination and analysis at 2.0 A resolution Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.180 0.013 0.014 isovaleryl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 0.013 0.021 hexanoyl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 0.019
-
 acetyl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 0.02 0.022 butyryl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 0.024 0.037 isobutyryl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.180 36000
-
 2 * 36000, recombinant enzyme, SDS-PAGE and gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Staphylococcus aureus
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Staphylococcus aureus
-
-
-
2.3.1.180 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.180 recombinant His2-tagged enzyme from Escherichia coli by nickel and Blue Sepharose affinity chromatography, and gel filtration Staphylococcus aureus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 ping pong reaction mechanism, catalytically important residues are Phe298 and His238 Staphylococcus aureus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.180 0.000001
-
 purified recombinant enzyme, primer substrate acetyl-CoA Staphylococcus aureus
2.3.1.180 0.000003
-
 purified recombinant enzyme, primer substrate lauroyl-CoA Staphylococcus aureus
2.3.1.180 0.000005
-
 purified recombinant enzyme, primer substrate palmitoyl-CoA Staphylococcus aureus
2.3.1.180 0.000006
-
 purified recombinant enzyme, primer substrate 2-methylcrotonyl-CoA Staphylococcus aureus
2.3.1.180 0.000013
-
 purified recombinant enzyme, primer substrate propionyl-CoA Staphylococcus aureus
2.3.1.180 0.000015
-
 purified recombinant enzyme, primer substrate isovaleryl-CoA Staphylococcus aureus
2.3.1.180 0.000022
-
 purified recombinant enzyme, primer substrate butyryl-CoA Staphylococcus aureus
2.3.1.180 0.000024
-
 purified recombinant enzyme, primer substrate hexanoyl-CoA Staphylococcus aureus
2.3.1.180 0.000031
-
 purified recombinant enzyme, primer substrate isobutyryl-CoA Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 additional information rank of activity in decreasing order: isobutyryl-CoA, hexanoyl-CoA, butyryl-CoA, isovaleryl-CoA, acetyl-CoA Staphylococcus aureus ?
-
 ?
2.3.1.180 2-methylcrotonyl-CoA + malonyl-[acyl-carrier protein]
-
 Staphylococcus aureus (4E)-4-methylhex-4-enoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Staphylococcus aureus acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] low activity Staphylococcus aureus acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 butyryl-CoA + malonyl-[acyl-carrier protein]
-
 Staphylococcus aureus 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 hexanoyl-CoA + malonyl-[acyl-carrier protein] high activity Staphylococcus aureus 3-oxo-octanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 isobutyryl-CoA + malonyl-[acyl-carrier protein] isobutyryl-CoA is the preferred primer substrate Staphylococcus aureus 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 isovaleryl-CoA + malonyl-[acyl-carrier protein]
-
 Staphylococcus aureus 3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 lauroyl-CoA + malonyl-[acyl-carrier protein]
-
 Staphylococcus aureus 3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information the enzyme possesses a large primer binding pocket determining the substrate specificity Staphylococcus aureus ?
-
 ?
2.3.1.180 palmitoyl-CoA + malonyl-[acyl-carrier protein]
-
 Staphylococcus aureus 3-oxo-stearoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 propionyl-CoA + malonyl-[acyl-carrier protein]
-
 Staphylococcus aureus 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.180 dimer 2 * 36000, recombinant enzyme, SDS-PAGE and gel filtration Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Staphylococcus aureus
2.3.1.180 FabH
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 30
-
 assay at Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.180 3.13
-
 acetyl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 3.8
-
 butyryl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 4.4 7.2 acetyl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 8.1
-
 hexanoyl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 15.5 30.5 isovaleryl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 20.9 41.6 butyryl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 25.5 41.7 hexanoyl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus
2.3.1.180 28 47.1 isobutyryl-CoA pH 7.0, 30°C, recombinant enzyme Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7
-
 assay at Staphylococcus aureus