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Literature summary extracted from

  • Kim, A.R.; Doherty-Kirby, A.; Lajoie, G.; Rylett, R.J.; Shilton, B.H.
    Two methods for large-scale purification of recombinant human choline acetyltransferase (2005), Protein Expr. Purif., 40, 107-117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.6 large-scale expression as His-tag or chitin-binding-domain fusion protein in Escherichia coli, His-tag fusion protein expresses in inclusion bodies at 37°C growth temperature and in soluble form at 20°C, chitin-binding-domain fusion protein expressed at 15°C but not at 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.6 acetyl-CoA + choline Homo sapiens synthesis of the neurotransmitter acetylcholine, deficiency in ChAT involved in several neurological and psychiatric disorders acetylcholine + CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.6 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.6 extremely sensitive to oxidation due to its cysteine-rich nature, oxidation causes formation of dimers and loss of activity Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.6 recombinant proteins using His-tag or chitin-binding-domain Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.6 brain
-
 Homo sapiens
-
2.3.1.6 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.6 50
-
 purified enzyme, pH 7.4 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.6 acetyl-CoA + choline
-
 Homo sapiens acetylcholine + CoA
-
 r
2.3.1.6 acetyl-CoA + choline synthesis of the neurotransmitter acetylcholine, deficiency in ChAT involved in several neurological and psychiatric disorders Homo sapiens acetylcholine + CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.6 monomer gel filtration, dimerization caused by oxidation can be reversed by addition of reducing agents Homo sapiens