EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.20.99.1 | purified wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, enzyme in 0.1 M sodium acetate, pH 4.4-4.6, 0.2 M ammonium sulfate, in presence of 30-35% PEG methyl ether, and 5 mM DTT, crystal structure in reduced form, X-ray diffraction structure determination and analysis at 1.6-2.4 A resolution | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.20.99.1 | arsenate + reduced acceptor | Bacillus subtilis | enzyme participates to achieve arsenate resistance to cells by reducing toxic arsenate to arsenite, arsenate is toxic due to its ability to mimic the phosphate group | arsenite + acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.20.99.1 | Bacillus subtilis | - |
gene arsC | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.20.99.1 | arsenite + acceptor + H2O = arsenate + reduced acceptor | catalytic mechanism involves the essential residues C10, C82, C89, R16, and D105, triple cysteine redox relay reduction mechanism, active site structure | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.20.99.1 | arsenate + reduced acceptor | - |
Bacillus subtilis | arsenite + acceptor | - |
? | |
1.20.99.1 | arsenate + reduced acceptor | enzyme participates to achieve arsenate resistance to cells by reducing toxic arsenate to arsenite, arsenate is toxic due to its ability to mimic the phosphate group | Bacillus subtilis | arsenite + acceptor | - |
? | |
1.20.99.1 | additional information | the enzyme also catalyzes the tyrosine phosphatase reaction in vitro dephosphorylizing e.g. 4-nitrophenyl phosphate | Bacillus subtilis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.20.99.1 | arsenate reductase | - |
Bacillus subtilis |