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Literature summary extracted from

  • Leduc, D.; Graziani, S.; Lipowski, G.; Marchand, C.; Le Marechal, P.; Liebl, U.; Myllykallio, H.
    Functional evidence for active site location of tetrameric thymidylate synthase X at the interphase of three monomers (2004), Proc. Natl. Acad. Sci. USA, 101, 7252-7257.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.148 H48Q inactive mutant enzyme Helicobacter pylori
2.1.1.148 H48Q/S84A inactive mutant enzyme Helicobacter pylori
2.1.1.148 H48Q/S84C inactive mutant enzyme Helicobacter pylori
2.1.1.148 R74A the KM-value for dUMP is 5.1fold higher than the wild-type value, the turnover-number is 4.6fold lower than the wild-type value Helicobacter pylori
2.1.1.148 R74K the KM-value for dUMP is nearly identical to wild-type value, the turnover-number is 6.6fold lower than the wild-type value Helicobacter pylori
2.1.1.148 S84A the KM-value for dUMP is 5.3fold higher than the wild-type value, the turnover-number is 2.1fold lower than the wild-type value. Mutation abolishes thymidylate synthase activity in vivo Helicobacter pylori
2.1.1.148 S84A/S85A inactive mutant enzyme Helicobacter pylori
2.1.1.148 S84C mutation abolishes thymidylate synthase activity in vivo Helicobacter pylori
2.1.1.148 S84Y the KM-value for dUMP is 1.9fold higher than the wild-type value, the turnover-number is 1.6fold lower than the wild-type value Helicobacter pylori
2.1.1.148 Y87F the KM-value for dUMP is 1.8fold lower than the wild-type value, the turnover-number is nearly identical to the wild-type value Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.148 additional information functional evidence for active site location of tetrameric thymidylate synthase X at the interphase of three monomers. The active-site configurations of ThyX proteins, present in many human pathogenic bacteria, and of human thymidylate synthase A (EC 2.1.1.45) are different. Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.148 0.00083
-
 dUMP mutant enzyme S84C Helicobacter pylori
2.1.1.148 0.0012
-
 dUMP mutant enzyme R74K Helicobacter pylori
2.1.1.148 0.0017
-
 dUMP mutant enzyme R74A Helicobacter pylori
2.1.1.148 0.0037
-
 dUMP mutant enzyme S84A Helicobacter pylori
2.1.1.148 0.0076
-
 dUMP wild-type enzyme Helicobacter pylori
2.1.1.148 0.008
-
 dUMP mutant enzyme Y87F Helicobacter pylori
2.1.1.148 0.012
-
 dUMP mutant enzyme S84Y Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.148 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.148
-
 Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Helicobacter pylori dTMP + tetrahydrofolate + FAD
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.148 ? x * 27168.2, wild-type enzyme, MALDI-TOF Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.1.1.148 thymidylate synthase X
-
 Helicobacter pylori
2.1.1.148 ThyX
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.148 0.0038
-
 dUMP mutant enzyme Y87F Helicobacter pylori
2.1.1.148 0.0072
-
 dUMP wild-type enzyme Helicobacter pylori
2.1.1.148 0.0078
-
 dUMP mutant enzyme R74K Helicobacter pylori
2.1.1.148 0.0097
-
 dUMP mutant enzyme S84Y Helicobacter pylori
2.1.1.148 0.037
-
 dUMP mutant enzyme R74A Helicobacter pylori
2.1.1.148 0.0384
-
 dUMP mutant enzyme S84A Helicobacter pylori
2.1.1.148 0.14
-
 dUMP mutant enzyme S84C Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.148 FAD wild-type and mutant enzymes Y87F, S84Y, H48Q, S84A and S84C contain quantities of tightly bound FAD. Mutant enzymes R74A, R74K, H48Q/S84A and H48Q/S84C easily lose considerable amounts of bound FAD Helicobacter pylori