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Literature summary extracted from

  • Karr, D.B.; Oehrle, N.W.; Emerich, D.W.
    Recovery of nitrogenase from aerobically isolated soybean nodule bacteroids (2003), Plant Soil, 257, 27-33.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.18.6.1 additional information several organic acids highly stimulate the enzyme activity of anaerobically isolated bacteroids, only malate has little effect Bradyrhizobium japonicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O Bradyrhizobium japonicum reduction of atmospheric dinitrogen to ammonium oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Bradyrhizobium japonicum
-
 strains 110 or 2134, anaerobically and aerobically isolated bacteroid from nodules of soybean
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.18.6.1 enzyme is extremely oxygen-labile Bradyrhizobium japonicum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.18.6.1 recovery of enzyme activity from aerobically isolated soybean nodules by incubation at low O2 pressure for about 1 h, recovery of activity can be inhibited by chloramphenicol and puromycin, and by spectinomycin only in strain 2134, not in strain 110, cycloheximide, actinomycin D, nalidixic acid, and rifampicin have no effect on enzyme activity recovery in strain 110, and cycloheximide, actinomycin D, and streptomycin have no effect on enzyme activity recovery in strain 2134 Bradyrhizobium japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 acetylene + ?
-
 Bradyrhizobium japonicum ethylene + ?
-
 ?
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O
-
 Bradyrhizobium japonicum oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ir
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O reduction of atmospheric dinitrogen to ammonium Bradyrhizobium japonicum oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ir

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.6.1 ATP
-
 Bradyrhizobium japonicum
1.18.6.1 Ferredoxin
-
 Bradyrhizobium japonicum