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Literature summary extracted from

  • Decottignies, P.; Flesch, V.; Gerard-Hirne, C.; Le Marechal, P.
    Role of positively charged residues in Chlamydomonas reinhardtii ferredoxin-NADP+-reductase (2003), Plant Physiol. Biochem., 41, 637-642.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expression in Escherichia coli, no posttranslational modification of the recombinant enzyme, the recombinant enzyme shows 26% of native enzyme activity Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.2 K135L site-directed mutagenesis, altered tertiary structure compared to the wild-type enzyme, no methylation at K83, 10% of native wild-type enzyme activity Chlamydomonas reinhardtii
1.18.1.2 K83L site-directed mutagenesis, altered tertiary structure compared to the wild-type enzyme, no methylation at K83, 2-4% of native wild-type enzyme activity Chlamydomonas reinhardtii
1.18.1.2 K83L/K89L site-directed mutagenesis, altered tertiary structure compared to the wild-type enzyme, no methylation at K83, 0.3% of native wild-type enzyme activity Chlamydomonas reinhardtii
1.18.1.2 K89L site-directed mutagenesis, altered tertiary structure compared to the wild-type enzyme, no methylation at K83, 4% of native wild-type enzyme activity Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 additional information
-
 additional information kinetics Chlamydomonas reinhardtii
1.18.1.2 0.0026
-
 reduced ferredoxin native enzyme, pH 8.0, 30°C Chlamydomonas reinhardtii
1.18.1.2 0.0066
-
 reduced ferredoxin recombinant enzyme, pH 8.0, 30°C Chlamydomonas reinhardtii
1.18.1.2 0.007
-
 NADPH native enzyme, pH 8.0, 30°C Chlamydomonas reinhardtii
1.18.1.2 0.033
-
 NADPH recombinant enzyme, pH 8.0, 30°C Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 36500
-
 native enzyme, gel filtration Chlamydomonas reinhardtii
1.18.1.2 37500
-
 native enzyme, native PAGE Chlamydomonas reinhardtii
1.18.1.2 38500
-
 recombinant enzyme, gel filtration Chlamydomonas reinhardtii
1.18.1.2 42000
-
 recombinant enzyme, native PAGE Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Chlamydomonas reinhardtii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.18.1.2 side-chain modification the native enzyme is posttranslationally trimethylated at lysine residues K83, K89, and K135 inducing changes in the tertiary enzyme structure Chlamydomonas reinhardtii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.1.2 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH positively charged residues K83 and K89 are required for catalytic activity, methylation of K83, K89, and K135 is required for optimal enzyme activity Chlamydomonas reinhardtii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 additional information
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2,6-dichlorophenolindophenol + NADPH + H+
-
 Chlamydomonas reinhardtii reduced 2,6-dichlorophenolindophenol + NADP+
-
 r
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase activity Chlamydomonas reinhardtii NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.2 reduced ferredoxin + NADP+ ferredoxin-dependent cytochrome c reduction, ferredoxin binding is independent of enzyme methylation status, positively charged residues K83 and K89 are required for activity Chlamydomonas reinhardtii oxidized ferredoxin + NADPH
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Chlamydomonas reinhardtii oxidized ferredoxin + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer
-
 Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+-reductase
-
 Chlamydomonas reinhardtii
1.18.1.2 ferredoxin-NADP-reductase
-
 Chlamydomonas reinhardtii
1.18.1.2 FNR
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.2 30
-
 assay at Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 35
-
 2,6-dichlorophenolindophenol native enzyme, pH 8.0, 30°C Chlamydomonas reinhardtii
1.18.1.2 37
-
 2,6-dichlorophenolindophenol recombinant enzyme, pH 8.0, 30°C Chlamydomonas reinhardtii
1.18.1.2 90
-
 reduced ferredoxin native enzyme, pH 8.0, 30°C Chlamydomonas reinhardtii
1.18.1.2 174
-
 reduced ferredoxin recombinant enzyme, pH 8.0, 30°C Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 8
-
 assay at Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 NADP+
-
 Chlamydomonas reinhardtii
1.18.1.2 NADPH
-
 Chlamydomonas reinhardtii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.18.1.2 Chlamydomonas reinhardtii native and recombinant enzyme, isoelectric focusing
-
 5.8