BRENDA - Enzyme Database show

Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase

Turner, G.W.; Croteau, R.; Plant Physiol. 136, 4215-4227 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.3.1.81
expression in Escherichia coli strain BL21(DE3)
Mentha x piperita
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.1.81
soluble
-
Mentha x piperita
-
-
1.14.14.51
endoplasmic reticulum
-
Mentha spicata
5783
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.1.81
38000
-
x * 38000
Mentha x piperita
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.81
2 (+)-pulegone + 2 NADPH + 2 H+
Mentha x piperita
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.81
Mentha x piperita
-
cv Black Mitcham
-
1.14.14.51
Mentha spicata
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.1.81
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision
Mentha x piperita
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.81
secretory trichome
secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development
Mentha x piperita
-
1.14.14.51
trichome
secretory cells of peltate glandular trichomes
Mentha spicata
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.81
2 (+)-pulegone + 2 NADPH + 2 H+
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
660251
Mentha x piperita
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
-
?
1.3.1.81
2 (+)-pulegone + 2 NADPH + 2 H+
the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio
660251
Mentha x piperita
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
-
?
1.14.14.51
(-)-(S)-limonene + NADPH + O2
-
660251
Mentha spicata
(-)-trans-carveol + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.1.81
?
x * 38000
Mentha x piperita
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.81
NADPH
dependent on
Mentha x piperita
1.14.14.51
NADPH
-
Mentha spicata
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.81
expression in Escherichia coli strain BL21(DE3)
Mentha x piperita
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.81
NADPH
dependent on
Mentha x piperita
1.14.14.51
NADPH
-
Mentha spicata
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.1.81
soluble
-
Mentha x piperita
-
-
1.14.14.51
endoplasmic reticulum
-
Mentha spicata
5783
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.1.81
38000
-
x * 38000
Mentha x piperita
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.81
2 (+)-pulegone + 2 NADPH + 2 H+
Mentha x piperita
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.81
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision
Mentha x piperita
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.81
secretory trichome
secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development
Mentha x piperita
-
1.14.14.51
trichome
secretory cells of peltate glandular trichomes
Mentha spicata
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.81
2 (+)-pulegone + 2 NADPH + 2 H+
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
660251
Mentha x piperita
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
-
?
1.3.1.81
2 (+)-pulegone + 2 NADPH + 2 H+
the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio
660251
Mentha x piperita
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
-
?
1.14.14.51
(-)-(S)-limonene + NADPH + O2
-
660251
Mentha spicata
(-)-trans-carveol + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.1.81
?
x * 38000
Mentha x piperita