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Literature summary extracted from

  • Turner, G.W.; Croteau, R.
    Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase (2004), Plant Physiol., 136, 4215-4227.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.81 expression in Escherichia coli strain BL21(DE3) Mentha x piperita

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.81 soluble
-
Mentha x piperita
-
-
1.14.14.51 endoplasmic reticulum
-
Mentha spicata 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.81 38000
-
x * 38000 Mentha x piperita

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.81 2 (+)-pulegone + 2 NADPH + 2 H+ Mentha x piperita the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview (-)-menthone + (+)-isomenthone + 2 NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.81 Mentha x piperita
-
cv Black Mitcham
-
1.14.14.51 Mentha spicata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.81 recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision Mentha x piperita

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.81 secretory trichome secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development Mentha x piperita
-
1.14.14.51 trichome secretory cells of peltate glandular trichomes Mentha spicata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.81 2 (+)-pulegone + 2 NADPH + 2 H+ the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview Mentha x piperita (-)-menthone + (+)-isomenthone + 2 NADP+
-
?
1.3.1.81 2 (+)-pulegone + 2 NADPH + 2 H+ the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio Mentha x piperita (-)-menthone + (+)-isomenthone + 2 NADP+
-
?
1.14.14.51 (-)-(S)-limonene + NADPH + O2
-
Mentha spicata (-)-trans-carveol + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.3.1.81 ? x * 38000 Mentha x piperita

Synonyms

EC Number Synonyms Comment Organism
1.3.1.81 More the enzyme is a member of the medium-chain dehydrogenase/reductase superfamily Mentha x piperita
1.3.1.81 pulegone reductase
-
Mentha x piperita
1.14.14.51 (-)-(4S)-limonene-6-hydroxylase
-
Mentha spicata
1.14.14.51 4S-limonene-6-hydroxylase
-
Mentha spicata
1.14.14.51 limonene-6-hydroxylase
-
Mentha spicata

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.81 NADPH dependent on Mentha x piperita
1.14.14.51 NADPH
-
Mentha spicata