EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.81 | expression in Escherichia coli strain BL21(DE3) | Mentha x piperita |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.1.81 | soluble | - |
Mentha x piperita | - |
- |
1.14.14.51 | endoplasmic reticulum | - |
Mentha spicata | 5783 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.81 | 38000 | - |
x * 38000 | Mentha x piperita |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.81 | 2 (+)-pulegone + 2 NADPH + 2 H+ | Mentha x piperita | the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.81 | Mentha x piperita | - |
cv Black Mitcham | - |
1.14.14.51 | Mentha spicata | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.81 | recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision | Mentha x piperita |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.81 | secretory trichome | secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development | Mentha x piperita | - |
1.14.14.51 | trichome | secretory cells of peltate glandular trichomes | Mentha spicata | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.81 | 2 (+)-pulegone + 2 NADPH + 2 H+ | the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview | Mentha x piperita | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? | |
1.3.1.81 | 2 (+)-pulegone + 2 NADPH + 2 H+ | the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio | Mentha x piperita | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? | |
1.14.14.51 | (-)-(S)-limonene + NADPH + O2 | - |
Mentha spicata | (-)-trans-carveol + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.81 | ? | x * 38000 | Mentha x piperita |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.81 | More | the enzyme is a member of the medium-chain dehydrogenase/reductase superfamily | Mentha x piperita |
1.3.1.81 | pulegone reductase | - |
Mentha x piperita |
1.14.14.51 | (-)-(4S)-limonene-6-hydroxylase | - |
Mentha spicata |
1.14.14.51 | 4S-limonene-6-hydroxylase | - |
Mentha spicata |
1.14.14.51 | limonene-6-hydroxylase | - |
Mentha spicata |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.81 | NADPH | dependent on | Mentha x piperita | |
1.14.14.51 | NADPH | - |
Mentha spicata |