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Literature summary extracted from

  • Nakajima, M.; Sakamoto, T.; Wada, K.
    The complete purification and characterization of three forms of ferredoxin-NADP+ oxidoreductase from a thermophilic cyanobacterium Synechococcus elongatus (2002), Plant Cell Physiol., 43, 484-493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 gene petH, DNA and amino acid sequence determination and analysis Synechococcus elongatus

General Stability

EC Number General Stability Organism
1.18.1.2 complex formation with phycocyanin via the CpcD-like domain stabilizes the enzyme Synechococcus elongatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 34000
-
 x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus
1.18.1.2 43415
-
 x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus
1.18.1.2 45000
-
 x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus
1.18.1.2 78000
-
 x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Synechococcus elongatus Q93RE3 i.e. Thermosynechococcus elongatus, single copy gene petH
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.18.1.2 proteolytic modification dissociation of the enzyme-phycocyanin complex induces the specific proteolysis between the CpcD-like domain and the FAD-binding domain to give rise to the 34 kDa enzyme form Synechococcus elongatus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 34 kDa enzyme form, 78 kDa complexed enzyme by cation exchange chromatography, and gel filtration, active 45 kDa enzyme form to homogeneity by ammonium sulfate precipitation, DEAE ion exchange chromatography, and gel filtration Synechococcus elongatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 additional information
-
-
 Synechococcus elongatus
1.18.1.2 11
-
 purified 45 kDa enzyme form, substrate 2,6-dichlorophenolindophenol, 22°C Synechococcus elongatus

Storage Stability

EC Number Storage Stability Organism
1.18.1.2 -30°C, aggregation of the 45 kDa enzyme form occurs during storage, 50% loss of activity after 1 week Synechococcus elongatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase activity Synechococcus elongatus NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Synechococcus elongatus oxidized ferredoxin + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.2 ? x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ oxidoreductase
-
 Synechococcus elongatus
1.18.1.2 FNR
-
 Synechococcus elongatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.2 additional information
-
 temperature dependence, overview Synechococcus elongatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.18.1.2 25 70 activity at 52°C is 3fold higher than activity at 25°C Synechococcus elongatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.18.1.2 70
-
 5 min, completely stable Synechococcus elongatus
1.18.1.2 80
-
 5 min, loss of 60% activity Synechococcus elongatus
1.18.1.2 85
-
 5 min, inactivation Synechococcus elongatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 8
-
 assay at Synechococcus elongatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD
-
 Synechococcus elongatus
1.18.1.2 NADP+
-
 Synechococcus elongatus