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Literature summary extracted from

  • Kakimoto, T.
    Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases (2001), Plant Cell Physiol., 42, 677-685.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.112 expressed in Escherichia coli AD494(DE3)pLysS cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.27 0.0065
-
 dimethylallyl diphosphate AtIPT4 Arabidopsis thaliana
2.5.1.27 0.018
-
 ATP AtIPT4 Arabidopsis thaliana
2.5.1.112 0.0065
-
 dimethylallyl diphosphate in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C Arabidopsis thaliana
2.5.1.112 0.018
-
 ATP in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.112 dimethylallyl diphosphate + ADP Arabidopsis thaliana
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP Arabidopsis thaliana highest activity with ATP diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Arabidopsis thaliana Q94ID3 AtIPT1
-
2.5.1.27 Arabidopsis thaliana Q94ID3 AtIPT4
-
2.5.1.112 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.112 Ni-NTA agarose column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.112 callus
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + ADP the enzyme preferrs ADP and ATP rather than AMP Arabidopsis thaliana dimethylallyl-ADP + diphosphate
-
 ?
2.5.1.27 dimethylallyl diphosphate + AMP the enzyme preferrs ADP and ATP rather than AMP Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
 ?
2.5.1.27 dimethylallyl diphosphate + ATP the enzyme preferrs ADP and ATP rather than AMP Arabidopsis thaliana dimethylallyl-ATP + diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ADP
-
 Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP highest activity with ATP Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
2.5.1.112 additional information no activity with AMP and tRNA Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.27 AtIPT1
-
 Arabidopsis thaliana
2.5.1.27 AtIPT4
-
 Arabidopsis thaliana
2.5.1.27 DMAPP:ATP/ADP isppentenyltransferase
-
 Arabidopsis thaliana
2.5.1.112 dimethylallyl diphosphate:ATP/ADP isopentenyltransferase
-
 Arabidopsis thaliana
2.5.1.112 IPT4
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.112 ADP
-
 Arabidopsis thaliana
2.5.1.112 ATP
-
 Arabidopsis thaliana