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Literature summary extracted from

  • Sakano, Y.; Okada, Y.; Matsunaga, A.; Suwama, T.; Kaneko, T.; Ito, K.; Noguchi, H.; Abe, I.
    Molecular cloning, expression, and characterization of adenylate isopentenyltransferase from hop (Humulus lupulus L.) (2004), Phytochemistry, 65, 2439-2446.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.27 expression in Escherichia coli Humulus lupulus
2.5.1.112 expressed in Escherichia coli M15[pREP4] cells Humulus lupulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.27 0.0162
-
 ATP pH 8.0, 25°C Humulus lupulus
2.5.1.27 0.0193
-
 ADP pH 8.0, 25°C Humulus lupulus
2.5.1.27 0.0195
-
 dimethylallyl diphosphate pH 8.0, 25°C Humulus lupulus
2.5.1.27 0.759
-
 AMP pH 8.0, 25°C Humulus lupulus
2.5.1.112 0.0162
-
 ATP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 0.0193
-
 ADP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 0.0195
-
 dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 0.759
-
 AMP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.27 Mg2+ dependent on Humulus lupulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.27 37000
-
 x * 37000, SDS-PAGE Humulus lupulus
2.5.1.112 36693
-
 x * 36693, calculated from amino acid sequence Humulus lupulus
2.5.1.112 37000
-
 x * 37000, SDS-PAGE Humulus lupulus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.112 dimethylallyl diphosphate + ADP Humulus lupulus
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP Humulus lupulus ATP is the best cosubstrate diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Humulus lupulus Q5GHF7
-
-
2.5.1.112 Humulus lupulus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.27
-
 Humulus lupulus
2.5.1.112 Ni-chelate affinity column chromatography Humulus lupulus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.27 cone
-
 Humulus lupulus
-
2.5.1.112 cone
-
 Humulus lupulus
-
2.5.1.112 leaf
-
 Humulus lupulus
-
2.5.1.112 root
-
 Humulus lupulus
-
2.5.1.112 stem
-
 Humulus lupulus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + ADP the enzyme prefers ADP and ATP rather than AMP Humulus lupulus ?
-
 ?
2.5.1.27 dimethylallyl diphosphate + AMP the enzyme prefers ADP and ATP rather than AMP Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
 ?
2.5.1.27 dimethylallyl diphosphate + ATP the enzyme prefers ADP and ATP rather than AMP Humulus lupulus ?
-
 ?
2.5.1.27 additional information no activity with GMP, IMP, CMP and UMP Humulus lupulus ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + ADP
-
 Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + AMP low activity with AMP Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine phosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP ATP is the best cosubstrate Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
2.5.1.112 additional information GMP, IMP, CMP, and UMP are not accepted as a substrate Humulus lupulus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.27 ? x * 37000, SDS-PAGE Humulus lupulus
2.5.1.112 ? x * 37000, SDS-PAGE Humulus lupulus
2.5.1.112 ? x * 36693, calculated from amino acid sequence Humulus lupulus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.27 AIPT
-
 Humulus lupulus
2.5.1.112 adenylate isopentenyltransferase
-
 Humulus lupulus
2.5.1.112 AIPT
-
 Humulus lupulus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.27 0.00828
-
 AMP pH 8.0, 25°C Humulus lupulus
2.5.1.27 0.022
-
 ATP pH 8.0, 25°C Humulus lupulus
2.5.1.27 0.024
-
 ADP pH 8.0, 25°C Humulus lupulus
2.5.1.27 0.027
-
 dimethylallyl diphosphate pH 8.0, 25°C Humulus lupulus
2.5.1.112 0.008
-
 AMP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 0.022
-
 ATP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 0.024
-
 ADP in 100 mM Tris–HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 0.027
-
 dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.27 6 9 either in Tris-HCl or potassium-phosphate buffer Humulus lupulus

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.112 ADP preferred ligand over AMP Humulus lupulus
2.5.1.112 ATP preferred ligand over AMP Humulus lupulus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.112 0.0109
-
 AMP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 1.238
-
 ADP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 1.34
-
 ATP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
2.5.1.112 1.397
-
 dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus