Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Varshney, U.; Ramesh, V.; Madabushi, A.; Gaur, R.; Subramanya, H.S.; RajBhandary, U.L.
    Mycobacterium tuberculosis Rv2118c codes for a single-component homotetrameric m1A58 tRNA methyltransferase (2004), Nucleic Acids Res., 32, 1018-1027.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.220 expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.220 Mg2+ 1-10 mM, decrease in methylation rate. 95% inhibition at 10 mM Mycobacterium tuberculosis
2.1.1.220 additional information methylation at A58 is not affected by the presence of ribothymidine at position 54 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.220 31000
-
 4 * 31000, SDS-PAGE Mycobacterium tuberculosis
2.1.1.220 115000
-
 gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.220 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.220
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.220 S-adenosyl-L-methionine + adenine58 in tRNA specific for the A58 position. Rv2118p does not methylate other positions in tRNA to any significant extent Mycobacterium tuberculosis S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.220 homotetramer 4 * 31000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.220 m1A58 tRNA methyltransferase
-
 Mycobacterium tuberculosis
2.1.1.220 Rv2118p
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.220 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.220 8
-
 assay at Mycobacterium tuberculosis